Volume : 12, Issue : 08, August- 2025

Title:

REVERSE PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY METHOD FOR SIMULTANEOUS ESTIMATION OF BACLOFEN AND TRAMADOL IN BULK AND PHARMACEUTICAL DOSAGE FORM

Authors :

J. Shireesha, P. Aravinda Reddy

Abstract :

The present research work describes a novel, simple, accurate, sensitive, rapid reversed-phase liquid chromatographic method for simultaneous estimation of Baclofen and Tramadol in bulk & pharmaceutical formulations. The chromatographic separation was achieved on WATERS HPLC with PDA detector and column Inertsil ODS, phosphate buffer and methanol as mobile phase at a flow rate of 1.0ml/min. The detection was carried out at 256 nm. The retention time of Baclofen and Tramadol was found to be 2.5 and 3.9. %Recoveries obtained for Baclofen and Tramadol were 99.85% & 100.5%.The %RSD below 2.0 shows the high precision of proposed method. The method was validated for precision, Recovery, Specify Detection & Quantification limits in accordance with ICH guidelines.
KEYWORDS: Baclofen, Tramadol, RP-HPLC, Validation

Cite This Article:

Please cite this article in press P. Aravinda Reddy et al., Reverse Phase High Performance Liquid Chromatography Method For Simultaneous Estimation Of Baclofen And Tramadol In Bulk And Pharmaceutical Dosage Form., Indo Am. J. P. Sci, 2025; 12(08).

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