Volume : 12, Issue : 08, August- 2025
Title:
SIMULTANEOUS ESTIMATION OF TELMISARTAN AND AMLODIPINE BY UV SPECTROSCOPY
Authors :
Buggana Siva Jyothi*, Syeda Fathima Batool, Sumayya Maheen, Bhavani Singh
Abstract :
The present work represents a UV spectrophotometric method for the simultaneous estimation of Telmisartan and Amlodipine in bulk and combined tablet dosage form. The method was based on employing the simultaneous equation method for analysis of both drugs, Telmisartan and Amlodipine have shown absorbance maxima at 225nm and 210 nm in methanol. The analytical method was validated for various parameters as per ICH (International Conference on Harmonization) guidelines. The developed simultaneous equation method obeyed Beer-Lambert’s law in the concentration range of 4-40 μg/ml for Telmisartan and 0.5-5 μg/ml for Amlodipine. The slope, intercept and correlation coefficient values of telmisartan at 225nm are 0.008, 0.0002 and 0.999. The slope, intercept and correlation coefficient values of Amlodipine at 210nm are 0.0378,0.0137 and 0.999. Accuracy showed acceptable recovery of 90- 110%, as per ICH guidelines. Inter and Intraday precision showed good reproducibility and repeatability with %RSD <2%. Robustness was performed by varying wavelength and the considered method as robust with %RSD <2%. Ruggedness performed by different analysts and considered method as rugged with %RSD <2%. The LOD and LOQ were 0.0107μg/ml and 0.032 μg/ml for Telmisartan and 0.006μg/ml and 0.0183μg/ml for Amlodipine respectively. The developed method was successfully applied for the estimation of drug content in Telmisartan 40mg and Amlodipine 5mg Tablets I.P. Percentage assay of Telma®– AM tablet was found to be 98.3 and 99.94. Hence the proposed method was found to be simple, rapid, accurate and precise and can be applied for the routine quality control studies for assay of Telmisartan and Amlodipine in bulk and tablet dosage forms.
Keywords: UV spectrophotometry, Telmisartan, Amlodipine, Simultaneous equation method, and validation.
Cite This Article:
Please cite this article in press Buggana Siva Jyothi et al ., Simultaneous Estimation Of Telmisartan And Amlodipine By Uv Spectroscopy., Indo Am. J. P. Sci, 2025; 12(08).
Number of Downloads : 10
References:
1.Misturu Ohishi et al, Effect of a tablet with a combination of telmisartan and amlodipine on patients with hypertension, Hypertension Research, 2011, 13(1), 459-466
2. Deepali M. Dangrade et al, Absorbance correction method for the simultaneous estimation of hydrochlorthiazide, telmisartan and amlodipine besylate in API and combined tablet dosage formulation by UV spectrophotometry, Scholars Research Library, 2012, 3(4), 1202-1207.
3.Validation Of Analytical Procedures Q2(R2), International Council For Harmonization Of Technical Requirements For Pharmaceuticals, European Medicines Agency, 2022, 1-34.
4.Validation of analytical procedures: text and methodology Q2(R1), International Conference of Harmonization, Geneva, 2005, 1-30.
5. Nina Alizadeh et al., Spectrophotometric method for the determination of amlodipine besylate in pure and dosage forms using 7,7,8,8-tetracyanoquinodimethane and tetracyanoethylene, Bulletin of Faculty of Pharmacy, Cairo University, 2014, 5(2), 109–114.
6.PubChem Compound Summary for Telmisartan, National Center for Biotechnology Information, 2023 from https://pubchem.ncbi.nlm.nih.gov/compound/Telmisartan.
7. PubChem Compound Summary for Amlodipine, National Center for Biotechnology Information, 2023 from https://pubchem.ncbi.nlm.nih.gov/compound/Amlodipine.
8. Niranjan D. Chivate et al., Development of UV Spectrophotometric method for estimation and validation of Telmisartan as a pure API, Journal of pharmacy research, 2012, 5(6), 3331-3333.
9. Safila Naveed et al., Simple UV Spectrophotometric Assay of Amlodipine, American Journal of Chemistry and Application 2014, 1(4), 66-69.
10. Rajesh varma et al., UV spectroscopic method for estimation of amlodipine besylate in tablets, International Journal of Pharmaceutical, Chemical And Biological Sciences, 2014, 4(1), 69-73.
11.Sonali D. Rathod et al., UV spectrophotometric method for estimation of telmisartan in bulk and tablet dosage form, International Journal of Pharmaceutical Sciences and Research, 2012, 3(10), 3936-3939
12. Manish Kumar et al., Simultaneous Estimation of Amlodipine Besylate and Ramipril in Tablets Dosage Form by UV Spectrophotometric Method, Journal of Pharmaceutical Sciences & Research, 2019, 11(2), 667-670.
13. Sasidhar.R.L.C. et al., A validated UV spectrophotometric method for the simultaneous estimation of hydrochlorothiazide, amlodipine besylate and telmisartan in bulk and combined tablet dosage form, Der Pharmacia Lettre, 2014, 6(4), 449-455.
14. Hirpara. K. P. et al., UV-Spectrophotometric Determination for Simultaneous Estimation of Amlodipine Besylate and Telmisartan in Combination, Journal Of Pharmaceutical Science And Bioscientific Research, 2012, 2(3), 133-137.
15. Raut. K. S. et al, UV Spectrophotometric estimation of Amlodipine besylate and Telmisartan in Bulk drug and Dosage form by Multiwavelength Analysis, International Journal of Chemical and Technical Research, 2010, 3(11), 2647-2650.
16. Chavhan et al., UV Spectrophotometric Method Development And Validation For Telmisartan In Bulk And Tablet Dosage Form, Asian Journal Of Pharmaceutical And Clinical Research, 2013, 6(4), 19-21.