Volume : 12, Issue : 08, August- 2025

Title:

DEVELOPMENT AND VALIDATION OF RP-HPLC METHOD FOR LACIDIPINE IN BULK FORM AND MARKETED PHARMACEUTICAL DOSAGE FORM

Authors :

Parimala Suryan*, Harshita, Poojitha, Sindhu, Yuvraj, Jeevan

Abstract :

Abstract:
A new analytical, simple, rapid, precise, accurate and reproducible RP-HPLC method for estimation of Lacidipine in bulk form and Marketed Pharmaceutical Dosage forms. Separation of Lacidipine was successfully achieved on a Kromasil ODS C18 (4.6mm x 250mm, 5m) column in an isocratic mode of separation utilizing Acetonitrile: Methanol in the ratio of 60:40% v/v at a flow rate of 1.0 mL/min and the detection was carried out at 272nm. The method was validated according to ICH guidelines for linearity, sensitivity, accuracy, precision, specificity and robustness. The response was found to be linear in the drug concentration range of 10-50mcg/mL for Lacidipine. The correlation coefficient was found to be 0.999 for Lacidipine. The LOD and LOQ for Lacidipine were found to be 1.1µg/mL and 3.2µg/mL respectively. The proposed method was found to be good percentage recovery for Lacidipine, which indicates that the proposed method is highly accurate. The specificity of the method shows good correlation between retention times of standard solution with the sample solution. Therefore, the proposed method specifically determines the analyte in the sample without interference from excipients of pharmaceutical dosage forms.
Keywords: Lacidipine, RP-HPLC, Accuracy, Precision, Robustness, ICH Guidelines.

Cite This Article:

Please cite this article in press Parimala Suryan et al., Development And Validation Of Rp-Hplc Method For Lacidipine In Bulk Form And Marketed Pharmaceutical Dosage Form., Indo Am. J. P. Sci, 2025; 12(08).

Number of Downloads : 10

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