Volume : 12, Issue : 08, August- 2025
Title:
A VALIDATED RP-HPLC METHOD FOR THE SIMULTANEOUS ESTIMATION OF AMLODIPINE AND ATENOLOL IN BULK AND ITS PHARMACEUTICAL DOSAGE FORM
Authors :
Ms.G.Bhavani*,Mrs.K.Srivani,Mrs.N.Sandhya Rani
Abstract :
A rapid and precise reverse phase high performance liquid chromatographic method has been developed for the validated of Amlodipine and Atenolol, in its pure form as well as in tablet dosage form. Chromatography was carried out on a Phenomenex Gemini C18 (4.6 x 150mm, 5µm) column using a mixture of Methanol: TEA Buffer pH 4.5 (35:65) as the mobile phase at a flow rate of 1.0ml/min, the detection was carried out at 240 nm. The retention time of the Amlodipine and Atenolol was 2.256, 5.427 ±0.02min respectively. The method produce linear responses in the concentration range of 5-25µg/ml of Amlodipine and 50-250µg/ml of Atenolol. The method precision for the determination of assay was below 2.0%RSD. The method is useful in the quality control of bulk and pharmaceutical formulations.
Keywords Amlodipine, Atenolol, RP-HPLC, validation.
Cite This Article:
Please cite this article in press G.Bhavani et al., A Validated Rp-Hplc Method For The Simultaneous Estimation Of Amlodipine And Atenolol In Bulk And Its Pharmaceutical Dosage Form., Indo Am. J. P. Sci, 2025; 12(08).
Number of Downloads : 10
References:
1. Dr. Kealey and P.J Haines, Analytical Chemistry, 1stedition, Bios Publisher, (2002), PP 1-7.
2.Md. Ahsanul Haque1 , Asma Naznin1 , A.N.M Hamidul Kabir2 , Md. Khalid Hossain3 and S.M. Ashraful Islam1, Development and Validation of RP-HPLC Method for the Simultaneous Estimation of Atenolol and Amlodipine in Tablet Dosage Form, Dhaka Univ. J. Pharm. Sci. 9(2): 131-138, 2010 (December)
3.Prajwala naikini, anusha akula, a. Ajitha, dr. V. Uma maheswara rao, rp-hplc method development and validation for the simultaneous estimation of amlodipine and atenolol in bulk and tablet dosage forms, Received: 09 Oct 2013, Revised and Accepted: 01 Nov 2013.
4.Imam ibne wahed, robiul islam, alam khan, m. Belal hossain and bytul m rahman,simultaneous high-performance liquid chromatographic determination of atenolol and amlodipine in pharmaceutical-dosage form, Pak. J. Pharm. Sci., 2007, Vol.20(4), 274-279.
5.Blessen Philip, Juddy Joseph and M. Sundarapandian, Rp-Hplc Method Development And Validation For Simultaneous Estimation Of Atenolol And Amlodipine Besylate In Pharmaceutical Dosage Forms, Philip et al., IJPSR, 2011; Vol. 2(8): 2156-2161, Received on 29 April, 2011; received in revised form 14 July, 2011; accepted 23 July, 2011.
6.Rashid R. Munjewar1, Sayyed Hussain2 , Development And Validation Of A Simultaneous Hplc Method For Quantification Of Atenolol And Amlodipine Besylate In Tablets , Article Received on09 August 2014, Revised on 26 August 2014, Accepted on 20 September 2014 , Volume 3, Issue 10, 1011-1020.
7.A.BraithWait and F.J.Smith, Chromatographic Methods, 5thedition, Kluwer Academic Publisher, (1996), PP 1-2.
8.Andrea Weston and Phyllisr. Brown, HPLC Principle and Practice, 1st edition,Academic press, (1997), PP 24-37.
9.Yuri Kazakevich and Rosario Lobrutto, HPLC for Pharmaceutical Scientists, 1stedition, Wiley Interscience A JohnWiley & Sons, Inc., Publication, (2007), PP 15-23.
10.Chromatography, (online). URL:http://en.wikipedia.org/wiki/Chromatography. Introduction to Column. (Online),URL:http://amitpatel745.topcities.com/index_files/study/column care.pdf
11.Detectors (online),URL:http://www.chem.agilent.com/Library/applications/59643559.pdf
12.Data elements required for assay validation, (online) available from: URL: http://www.labcompliance.com/tutorial/methods/default.aspx.
14.Snyder LR practical HPLC method development, 2nd edition. John Wiley and sons, New York, (1997), PP 180-182.
13.ICH Q2A, “validation of analytical methods, definitions and terminology”, ICH Harmonized tripartite guideline, (1999).
14. Ezzati M, Lopez AD, Rodgers A, Vander Hoorn S, Murray CJ. Selected major risk factors and global and regional burden of disease, Lancet 2000; 360: 1347-60.
15. Chauhan A. A review on atenolol, World J Pharm Pharm Sci, 2020; 9(3): 902-914.
16. Abdussaleem K, Boopathy D, Perumal P. Analytical Method Development and Validation of Losartan Potassium and Atenolol in combined dosage form by RP-HPLC, Int.J PharmTech Res, 2010; 2(1): 471-474.