Volume : 12, Issue : 12, December- 2025
Title:
ISOLATION & CHARACTERIZATION OF BIOACTIVE COMPOUND FROM MARINE ALGAE SARGASSUM WIGHTII
Authors :
Avishra Farzeen*, Smita Jain, Dr. S. Nayak
Abstract :
Bioactive compound was isolated and characterized from selected marine algae Sargassum wightii and evaluated its antioxidant potential. Sargassum was dark brown algae having foul smell algae was 20-28cm in size and highly branched and leave size was 6-9 cm long and 3-10mm broad. Physiochemical screening of powdered fruit was done by the standard reported methods. The Loss on drying (0.2%), Total ash value (7%), Acid insoluble ash value (3.7%), Water soluble ash value (1.33) and Foaming index (8ml) was found. Extraction of algae Sargassum wightii was done by maceration extraction method. Obtained ethanolic extract was light brown in color, semisolid in consistency with 29.65% yield. The phytochemical analysis of the ethanolic extract of Sargassum wightii revealed the strong presence of phenolics and tannins, flavonoids and alkaloids. Total phenolic content was obtained. The phenolic content was expressed as gallic acid equivalence (GAE)/g of extract. The absorbance of all sample solutions was measured at 720 nm using a spectrophotometer. Total phenolic content was found as 0.835 GAE/g of extract.Column chromatography a brilliant technique for separating and purifying bioactive compounds from complex algal extracts of Sargassum wightii. The bioactive compounds present in the three different solvent extracts of Sargassum wightii were identified by GC-MS analysis. The compounds present in the extracts were identified after the comparison of the Mass Spectra with NIST Library. The identification of active principles was assured by observing their retention time (RT), molecular formula, molecular weight and peak area percentage. The compound having high peak area was collected from preparative TLC run at 0.57 Rf-value by spotting concentrated fraction eluted from column chromatography. Collected compound was identified as [Bicyclo [4.3.0] nonan-2-one, 8 isopropylidene]. Screen the bioactivity of isolated compounds using in-vitro DPPH assays and found higher percentage of inhibition (66.59%) by Eluted fraction from column. The 62.50 % inhibition was achieved at 500 μg/ml concentration of Ethanolic extract of Sargassum wightii.
KEYWORDS: Sargassum wightii, Marine, Bioactives, Isolation, Algae, Antioxidant
Cite This Article:
Please cite this article in press Avishra Farzeen et al., Isolation & Characterization Of Bioactive Compound From Marine Algae Sargassum Wightii, Indo Am. J. P. Sci, 2025; 12(12).
Number of Downloads : 10
References:
1. Leandro, A. Pacheco, D. Cotas, J. Marques, J.C. Pereira, L. Gonçalves, A.M.M. Seaweed’s Bioactive Candidate Compounds to Food Industry and Global Food Security. Life 2020, 10, 140.
2. Din, N.A.S. Mohd Alayudin, A.S. Sofian-Seng, N.S. Rahman, H.A. Mohd Razali, N.S. Lim, S.J. Wan Mustapha, W.A. Brown Algae as Functional Food Source of Fucoxanthin: A Review. Foods 2022, 11, 2235.
3. Cadar, E.; Axinte, E.R.; Amzoiu, M.; Jurja, S.; Cherim, M. Preliminary study on the marine algae from the romanian Black Sea coast. J. Sci. Arts 2019, 4, 989–1000.
4. Cadar, E.; Sirbu, R.; Negreanu-Pirjol, B.S.; Ionescu, A.-M.; Negreanu-Pirjol, T. Heavy metals bioaccumulation capacity on marine algae biomass from romanian Black Sea coast. Rev. Chim. 2019, 70, 3065–3072.
5. Cai, J. Global Status of Seaweed Production, Trade and Utilization; Food and Agriculture Organisation of United Nations: Rome, Italy, 2021; pp. 1–18.
6. André, R.; Pacheco, R.; Bourbon, M.; Serralheiro, M.L. Brown, Algae Potential as a Functional Food against Hypercholesterolemia: Review. Foods 2021, 10, 234.
7. Park, E.; Yu, H.; Lim, J.-H.; Choi, J.H.; Park, K.J.; Lee, J. Seaweed metabolomics: A review on its nutrients, bioactive compounds and changes in climate change. Food Res. Int. 2023, 163, 112221