21.Issue 12 december 25 - INDO AMERICAN JOURNAL OF PHARMACEUTICAL SCIENCES

Volume : 12, Issue : 12, December- 2025

Title:

REVIEW ON RP-HPLC METHODOLOGIES FOR SIMULTANEOUS ESTIMATION OF ANTIBACTERIAL DRUG LINEZOLID AND ITS IMPURITIES

Authors :

Jyosna Bhaskar Tayade, Miss. Madhuri A. Theng, Dr. Ashutosh Kumar Dash

Abstract :

Ensuring the quality, safety, and purity of antibacterial drugs requires robust analytical methods capable of detecting and quantifying both active pharmaceutical ingredients and their associated impurities. Reversed-phase high-performance liquid chromatography (RP-HPLC) remains the most reliable and widely adopted technique for this purpose due to its superior sensitivity, precision, and suitability for stability-indicating analysis. Linezolid, a vital oxazolidinone antibiotic used against multidrug-resistant Gram-positive pathogens, requires stringent impurity profiling because of its susceptibility to degradation under stress conditions. This review provides a conceptual overview of RP-HPLC method development and validation for antibacterial agents, highlighting key analytical parameters such as mobile phase selection, wavelength optimization, chromatographic behavior, and ICH-recommended validation criteria. The discussion also outlines comparative analytical considerations for Daptomycin and emphasizes current trends and future advancements in chromatographic technologies relevant to antibiotic quality control.
Keywords:
RP-HPLC; Linezolid; Impurity Profiling; Stability-Indicating Method; Method Validation; Antibacterial Analysis.

Cite This Article:

Please cite this article in press Jyosna Bhaskar Tayade et al., Review On Rp-Hplc Methodologies For Simultaneous Estimation Of Antibacterial Drug Linezolid And Its Impurities, Indo Am. J. P. Sci, 2025; 12(12).

Number of Downloads : 10

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