Volume : 12, Issue : 12, December- 2025
Title:
ADVANCES IN RADIOIMMUNOASSAY TECHNOLOGY: FROM CLASSICAL METHODS TO MODERN DIAGNOSTIC PLATFORMS
Authors :
Supriya Pintu Sawant*,Sakshi Sunil Kharade, Chaitrali Sandeep Kasture, Shashikant Shivanand Langote, Soyal D. Mujawar, Sabir Allauddin Bagwan, Nital Adnyan Dandage
Abstract :
Radioimmunoassay (RIA) is one of the most influential analytical techniques developed for the quantitative measurement of biologically active substances at extremely low concentrations. Since its introduction, RIA has revolutionized clinical diagnostics and biomedical research by enabling sensitive and specific detection of hormones, drugs, tumor markers, and infectious disease biomarkers. This review provides a comprehensive overview of the evolution of radioimmunoassay technology, beginning with classical competitive binding assays and progressing toward modernized and automated diagnostic platforms. Key aspects discussed include the fundamental principles of antigen–antibody interactions, commonly used radioisotopes and detection systems, method development and optimization strategies, and the wide-ranging clinical and research applications of classical RIA. The review also critically examines the limitations and safety concerns associated with radioactive materials, which have driven the transition toward non-radioactive immunoassay alternatives. Furthermore, recent technological advancements that have enhanced the sensitivity, automation, and sustainability of RIA are highlighted. Overall, this review emphasizes the enduring relevance of radioimmunoassay as a reference and specialized diagnostic tool, while acknowledging its pivotal role in shaping modern immunoassay technologies.
Keywords: Radioimmunoassay; Antigen–Antibody Interaction; Radiolabels; Immunoassay Evolution; Clinical Diagnostics; Analytical Sensitivity; Diagnostic Platforms
Cite This Article:
Please cite this article in press Supriya Pintu Sawant et al., Advances In Radioimmunoassay Technology: From Classical Methods To Modern Diagnostic Platforms, Indo Am. J. P. Sci, 2025; 12(12).
Number of Downloads : 10
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