Please cite this article in press Chirayu J.Sharma et al., A Comprehensive Review Article On Herbosomes .,Indo Am. J. P. Sci, 2025; 12 (01).

1. Varadkar M, Gadgoli C. Preparation and evaluation of wound healing activity of phytosomes of crocetin from Nyctanthes arbor-tristis in ratsJ. Tradit. Complement. Med. 2022;12(4):354-60.
2. Al-Rabia MW, Alhakamy NA, Rizg WY, Alghaith AF, Ahmed OA, Fahmy UA. Boosting curcumin activity against human prostatic cancer PC3 cells by utilizing scorpion venom conjugated phytosomes as promising functionalized nanovesicles. Drug Delivery. 2022;29(1):807-20.
3. Yadav et al, Design and Optimization of a Novel-Herbosomal Loaded PEG Poloxamer Topical Formulation For the Treatment of Cold Injuries: A Quality By Design Approach, Drug Deliv. Transl. Res., 2021, PREPRINT (Version 1).
4. Sari RK, Prayogo YH, Rozan SA, Rafi M, Wientarsih I. Antioxidant Activity, Sun Protection Activity, and Phytochemical Profile of Ethanolic Extracts of Daemonoropsacehensis Resin and Its Phytosomes. Sci. Pharm.2022;90(1):10.
5. Kumar S, Baldi A, Sharma DK. Characterization and In vitro investigation of antiscabietic effect of phytosomes assimilating quercetin and naringenin rich fraction of Pistaciaintegerrima galls extract against Sarcoptesscabiei. J Drug Deliv Sci Technol .2022;67:102-851.
6. Cheung M K.et al., Network pharmacology reveals potential functional components and underlying molecular mechanisms of Andrographis paniculata in esophageal cancer treatment,phytotherapy res. 2022:; 36(4) 1748-1760.
7. Alharbi WS, Almughem FA, Almehmady AM, Jarallah SJ, Alsharif WK, Alzahrani NM, Alshehri AA. Phytosomes as an emerging nanotechnology platform for the topical delivery of bioactive phytochemicals. Pharmaceutics. 2021;13(9):1475.
8. Sharma A. et al., Standardization of the indian crude drug kalmegh by high pressure liquid chromatographic determination of andrographolide, phytochemical analysis.1992; 3(3): 129-131.
9. Lee SY, Abdullah LC, Rahman RA, Abas F, Chong GH. Stability and toxicity profile of solution enhanced dispersion by supercritical fluids (SEDS) formulated Andrographis paniculata extract. Brazilian J. Chem. Eng. 2019;36:969-78.
10. Jain P, Taleuzzaman M, Kala C, Kumar Gupta D, Ali A, Aslam M. Quality by design (Qbd) assisted development of phytosomal gel of aloe vera extract for topical delivery. J. of Liposome Res. 2021;31(4):381-8.
11. Taleuzzaman M, Sartaj A, Kumar Gupta D, Gilani SJ, Mirza MA. Phytosomal gel of Manjistha extract (MJE) formulated and optimized with central composite design of Quality by Design (QbD). Journal J. Dispers. Sci. Technol. 2021;14:1-9.
12. Permana AD, Utami RN, Courtenay AJ, Manggau MA, Donnelly RF, Rahman L. An approach for enhanced both dissolution behaviour in biorelevant media and skin retention profiles. J. Photochem. Photobiol. B, Biol..2020;205:111846.
13. Alhakamy NA, Badr-Eldin SM, A. Fahmy U, Alruwaili NK, Awan ZA, Caruso G, Alfaleh MA, Alaofi AL, Arif FO, Ahmed OA, Alghaith AF. Thymoquinone-loaded soyphospholipid-based phytosomes exhibit anticancer potential against human lung cancer cells. Pharmaceutics. 2021;12(8):761.
14. Alhakamy NA, A. Fahmy U, Badr-Eldin SM, Ahmed OA, Asfour HZ, Aldawsari HM, Algandaby MM, Eid BG, Abdel-Naim AB, Awan ZA, K. Alruwaili N. Optimized icariinphytosomes exhibit enhanced cytotoxicity and apoptosis-inducing activities in ovarian cancer cells. Pharmaceutics. 2020;12(4):346.
15. Huang Z, Brennan CS, Zhao H, Liu J, Guan W, Mohan MS, Stipkovits L, Zheng H, Kulasiri D. Fabrication and assessment of milk phospholipid-complexed antioxidant phytosomes with vitamin C and E: A comparison with liposomes. Food chem… 2020;324:126837.
16. Kumar D, Vats N, Saroha K, Rana AC. Phytosomes as emerging nanotechnology for herbal drug delivery. In SustaiN. Agric. Reviews,2020; 43;217-237.
17. H. Shariare M, Afnan K, Iqbal F, A. Altamimi M, Ahamad SR, S. Aldughaim M, K. Alanazi F, Kazi M. Development and optimization of epigallocatechin-3-gallate (egcg) nanophytosome using design of experiment (DoE) and their in vivo anti-inflammatory studies. Molecules. 2020;25(22):5453.
18. Karole, S., Gautam, G.K., & Gupta, S.K. (2019). Preparation and evaluation of phytosomes containing ethanolic extract of leaves of Bombaxceiba for hepatoprotective activity, pharma innovation 2019;8(2):22-26.
19. Lee S. Y. et al, studied that stability and toxicity profile of solution enhanced dispersion by supercritical fluids (SEDS) formulated Andrographis paniculata extract, Braz. J. Chem. Eng. 2019;36 (2),969-978.
20. Sachin K S, Adlin JinoNesalin J, Tamizh Mani T, “Preparation and Evaluation of Curcumin Phytosomes by Rotary Evaporation Method, Int. J. pharm. biomed. res., 2019; 6(1),29-34.
21. Li, F., Li, X. M., Sheng, D., Chen, S. R., Nie, X., Liu, Z., Wang, D., Zhao, Q., Wang, Y., Wang, Y., & Zhou, G. C. (2018). Discovery and preliminary SAR of 14-aryloxyandrographolide derivatives as antibacterial agents with immunosuppressant activity. RSC advances, 8(17), 9440–9456.
22. Dai, Y. et al. “Overview of pharmacological activities of Andrographis paniculata and its major compound andrographolide.” Crit. Rev. Food Sci. Nutr. 2019; 59(1): S17-S29.
23. El-Menshawa SF. Ali AA, Rabeb MA and Khalil NM, Nanosized soy phytosome-based thermogel as topical anti-obesity formulation: Int. J. Nanomedicine, 2018; 13: 307-318.
24. Ika R. Sutejo,antioxidant and hepatoprotective activity of garlic chives (allium tuberosum) ethanolic extract on doxorubicin-induced liver injured, Int. J. Pharm. Med. 2017; 6(1):20-23.
25. Rathee S. and Kamboj A. optimization and development of antidiabeticphytosomes by box-benhken design, J. Liposome Res., 2017; 28(2): 161-172.
26. Gahandule M, Jadhav SJ, Gadhave MV, Gaikwad DD. Formulation and development of hepato-protective buteamonosperma-phytosome. Int J Res Pharm Pharm Sci. 2016;1(4):21-7.
27. Sundaraganapathy,Leena P N, Development and Evaluation of Anti-Cancer Activity of Phytosome Formulated from the Root Extract of Clerodendronpaniculatum Linn, Int. J. Pharmacogn. Phytochem, 2016; 8(11); 1778-1781
28. Telange, D.R., Patil, A.T., Pethe, A.M., Fegade, H., Anand, S. and Dave, V.S.,. Formulation and characterization of an apigenin-phospholipid phytosome (APLC) for improved solubility, in vivo bioavailability, and antioxidant potential. European Journal of Pharmaceutical Sciences,2017; 108:36-49.
29. Panraksa P, Ramphan S, Khongwichit S, Smith DR. Activity of andrographolide against dengue virus. Antiviral research. 2017;139:69-78.
30. Sonam and Sahu AN. Development and characterization of hepatoprotective phytosomes of abutilon indicum and Piper Longum, Int. J. Pharm. Biol. Sci., 2015; 5(4): 97-106.
31. Asija S, Ahuja A, Asija R. Formulation and evaluation of prosopis cineraria (linn.) drucephospholoipid complex, Asian J Pharm Clin Res, 2015, 3(2): 301–305.
32. Bhupen K. and Malay K Das. Resveratrol-Phospholipid complexes (phytosomes) with improved physicochemical properties favourable for drug delivery via skin, World J. Pharm. Res. 2015; 4(5): 1497-1517.
33. Hooresfand, Z., Ghanbarzadeh, S.andHamishehkar, H. Preparation and Characterization of Rutin-loaded Nanophytosomes. Pharmaceutical Sciences, 2015; 21: 145-151.
34. Giorgio P., Paola B., Ponti V., and Novelli C.,, Clinical Usefulness of Oral Supplementation with Alpha-Lipoic Acid, Curcumin Phytosome, and B-Group Vitamins in Patients with Carpal Tunnel Syndrome Undergoing Surgical Treatment, Evid Based Complement Alternat Med. 2014;49(2):2–32.
35. Khan J, Alexander A, Saraf S, Saraf S. Luteolin–phospholipid complex: preparation, characterization and biological evaluation. Int J Pharm Pharm Res. 2014;66(10):1451-62.
36. Chua LS. Review on liver inflammation and antiinflammatory activity of Andrographis paniculata for hepatoprotection. Phytother Res. 2014;28(11):1589-98.
37. Kumar S., Chemistry and Biological Activities of Flavonoids: An Overview, The Scientific World Journal, 2013, 2013(16).
38. Belcaro, G., Ledda, A., Hu, S., Cesarone, M.R., Feragalli, B. and Dugall, M., Green select phytosome for borderline metabolic syndrome. Evid.-based Complement. Altern. Med., 2013;(2013):1-7.
39. Jain PK, Khurana N, Pounikar Y, Gajbhiye A, Kharya MD. Enhancement of absorption and hepatoprotective potential through soya-phosphatidylcholine-andrographolide vesicular system J Liposome Res,2014; 1(9):1532-2394,
40. Eidi, A., Mortazavi, P., Bazargan, M. and Zaringhalam, J., Hepatoprotective activity of cinnamon ethanolic extract against CCI4-induced liver injury in rats. EXCLI J, 2012; 11: 495.
41. Chellampillai B, Pawar AP. Improved bioavailability of orally administered andrographolide from pH-sensitive nanoparticles. Eur J Drug MetabPharmacokinet .
2011;35(3):123-9.
42. Cuomo, J., Appendino, G., Dern, A.S., Schneider, E., McKinnon, T.P., Brown, M.J., Togni, S. and Dixon, B.M., Comparative absorption of a standardized curcuminoid mixture and its lecithin formulation. J. Nat. Prod, 2011;74(4):664-669.
43. Gupta, N.K. and Dixit, V.K., Development and evaluation of vesicular system for curcumin delivery. Arch Dermatol Res, 2011;303(2):89-101.
44. Zaveri, M., Gajjar, H., Kanaki, N. and Patel, S., Preparation and evaluation of drug phospholipid complex for increasing transdermal penetration of phytoconstituents. Int. J. pharm. life sci, 2011;1(3): 80-93.
45. Kidd, P.M., Bioavailability and activity of phytosome complexes from botanical polyphenols: the silymarin, curcumin, green tea, and grape seed extracts. Altern Med Rev, 2009;14(3):226-46.
46. Di Pierro F, Menghi AB, Barreca A, Lucarelli M, Calandrelli A. GreenSelect (R) phytosome as an adjunct to a low-calorie diet for treatment of obesity: a clinical trial. Alternative Medicine Review. 2009;14(2):154.
47. Yen, F.L., Wu, T.H., Lin, L.T., Cham, T.M. and Lin, C.C.,Naringenin-loaded nanoparticles improve the physicochemical properties and the hepatoprotective effects of naringenin in orally-administered rats with CCl4-induced acute liver failure.
Pharmaceutical res., 2009; 26(4): 893-902.
48. Girish C, Koner BC, Jayanthi S, Ramachandra Rao K, Rajesh B, Pradhan SC. Hepatoprotective activity of picroliv, curcumin and ellagic acid compared to silymarin on paracetamol induced liver toxicity in mice. FundamClinPharmacol. 2009;23(6):735-45.
49. Yanyu X, Yunmei S, Zhipeng C, Qineng P. The preparation of silybin-phospholipid complex and the study on its pharmacokinetics in rats. Int J Pharm. 2006;307(1):77-82.
50. Mohamed NT, Attia A, Ali, Hepatoprotective activity of Allicin against carbon tetrachloride induced hepatic injury in rats, J. Biol. Sci.,2006;6(3):457-468.
51. Maiti, K., Mukherjee, K., Gantait, A., NazeerAhamed, H., Saha, B.P. and Kumar Mukherjee, P., 2005. Enhanced therapeutic benefit of quercetin phospholipid complex in carbon tetrachloride-induced acute liver injury in rats: a comparative study Iran. J.
Pharmacol. Ther, 2005; 4(2): 84-0.
52. Busby, A., Grange, L.L., Edwards, J. and King, J., The use of a silymarin/phospholipid compound as a fetoprotectant from ethanol-induced behavioral deficits. J. Herb. Pharmacother., 2002;2(1):39-47.
53. Shalini, S., Kumar, R., R., &Birendra, S. (2015). Antiproliferative effect of Phytosome complex of methanolic extract of Terminaliaarjuna bark on Human Breast Cancer Cell Lines (MCF-7). International Journal of Drug Development and Research, 7, 173–182.
54. Sabzichi, M., Hamishehkar, H., Ramezani, F., Sharifi, S., Tabasinezhad, M., Pirouzpanah, M., Ghanbari, P., &Samadi, N. (2014). Luteolin-loaded phytosomes sensitize human breast carcinoma MDA-MB 231 cells to doxorubicin by suppressing Nrf2 mediated signalling. Asian Pacific Journal of Cancer Prevention: APJCP, 15(13), 5311–5316.
55. Yadav, R., B., Pathak, D., P., Varshney, R., &Arora, R. (2021). Design and optimization of a novel-Herbosomal loaded PEG-poloxamer topical formulation for the treatment of cold injuries: A quality by design approach.
56. Das, M. K., &Kalita, B. (2014). Design and evaluation of phyto-phospholipid complexes (phytosomes) of Rutin for transdermal application. Journal of Applied Pharmaceutical Science, 4(10), 51–57.
57. Bombardelli, Patri, P. (1988). Complexes of Saponins with Phosphlipids and Pharmaceutical and Cosmetic Compositions Containing Them, 9.
58. Mazumder, A., Du Dwivedi, A., Preez, J., L., Du, P., & J. (2016). In-vitro wound healing and cytotoxic effects of sinigrin-phytosome complex. International Journal of Pharmacy, 498, 283–293.
59. Devi, Lakshmi, S. Divakar, M., C. (2012). Wound healing activity studies of Wrightiaarboreaphytosome in rats. Hygeia J. drugs. Medico, 4, 87–94. 67. Zhang, &Jingze, Gao, Wenyuan, Bai, Shufang, Chen, Hong, Qiang, Qianqian, Liu, Z. 2011. Glycyrrhizic acid– phospholipid complex: preparation process optimization and therapeutic and pharmacokinetic evaluation in rats. Lat. Am. J. Pharm. 30, 1621–1630.
60. Gupta, N. K., & Dixit, V. K. (2011). Bioavailability enhancement of curcumin by complexation with phosphatidyl choline. Journal of Pharmaceutical Sciences, 100(5), 1987–1995.
61. Singh, D., Rawat, M., S., M., Semalty, A., &Semalty, M. (2012). Chrysophanol– phospholipid complex. Journal of Thermal Analysis and Calorimetry, 1–9.
62. Li, Y., Yang, D., J., Chen, S., L., Chen, S., B., Chan, A., & S. (2008). Process parameters and morphologyinpuerarin, phospholipids and their complex microparticles generation by super critical antisolvent precipitation. International Journal of Pharmacy, 359, 35–45.
63. Maiti, K., Mukherjee, K., Gantait, A., Ahamed, H., N., Saha, B., P., Ahamed, P., & K. (2005). Enhanced therapeutic benefit of quercetin–phospholipid complex in carbon tetrachloride-induced acute liver injury in rats: A comparative study. Iranian Journal of Pharmacology and Therapeutics, 4, 84–90.
64. Semalty, A., Semalty, M., Singh, D., &Rawat, M. S. M. (2010). Preparation and characterization of phospholipid complexes of naringenin for effective drug delivery. Journal of Inclusion Phenomena and Macrocyclic Chemistry, 67(3–4), 253–260.
65. Peng, Q., Gong, T., Zuo, J., Liu, J., Zhao, D., & Zhang, Z. (2008). Enhanced oral bioavailability of salvianolic acid B by phospholipid complex loaded nanoparticles. Pharmazie, 63(9), 661–666.
66. Sonam and Sahu AN. Development and characterization of hepatoprotective phytosomes of abutilon indicum and Piper Longum, Int. J. Pharm. Biol. Sci., 2015; 5(4): 97-106.