Volume : 12, Issue : 06, June – 2025
Title:
DEVELOPMENT AND VALIDATION OF RP-HPLC METHOD FOR QUANTITATIVE ANALYSIS OF LINEZOLID IN PURE AND PHARMACEUTICAL DOSAGE FORMS
Authors :
CH .Kantlam, Venkatesh*, B.Shiva Kumar Reddy, Deepak, E. Nandan Kumar, Farhan, Shravya
Abstract :
A novel, simple, accurate, precise, sensitive and specific analytical RP-HPLC method was developed and validated for the quantitative estimation of Linezolid in bulk drugs and pharmaceutical dosage form. Chromatographic separation was achieved on an Symmetry ODS C18 (4.6×250mm, 5µm) analytical column using mobile phase composition of Methanol and Phosphate Buffer in ratio of (35: 65% v/v) that was set at a flow rate of 1.0μl/min with detection of 235 nm. The retention time of Linezolid was found to be 3.006min. The drug was analyzed by following the guidelines of International conference on Harmonization (ICH). This drug showing linearity in the concentration range of 6-14µg/ml and the correlation coefficient showing R2 = 0.9996. The % Recoveries showing within the limits. The presentation of the method was validated according to the present ICH guidelines for accuracy, precision and robustness, Linearity, limit of quantification, limit of detection linearity.
Key Words: Linezolid, RP-HPLC, Method Development, Accuracy, Precision.
Cite This Article:
Please cite this article in press Ch.Kantlam et al., Development And Validation Of Rp-Hplc Method For Quantitative Analysis Of Linezolid In Pure And Pharmaceutical Dosage Forms., Indo Am. J. P. Sci, 2025; 12(07).
Number of Downloads : 10
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