Volume : 12, Issue : 06, June – 2025

Title:

IN VITRO ANTIOXIDANT EVALUATION OF FLOWRES OF EHRETIA LAEVIS ROXB BY DPPH RADICAL SCAVENGING ASSAY AND NIRIC OXIDE RADICAL SCAVENGING ASSAY

Authors :

Vinjavarapu L. Anusha*, B.Thangabalan*, CH.Keseena, Kishore, G.Lavanya, Y.Navya Reddy, D.Lakshami, M.Suleman, P. Lohit sai, N.Gopi krishna, B.Kalyani, Sk.Akthar Sahana, P.Sridhar

Abstract :

Ehretia laevis, a medicinal plant belonging to the boraginaceae family, is widely recognized for its therapeutic properties in traditional medicine. the present study aimed to explore the phytochemical profile and antioxidant potential of Ehretia laevis through hydro alcoholic extraction and solvent fractionation. The plant material was subjected to hydroalcoholic extraction (70% ethanol), followed by successive fraction into n-hexane, ethyl acetate, and tannins. Anti-oxidant activity was evaluated using DPPH (2, 2-diphenyl-1-picrylhydrazyl) and nitic oxide scavenging assays. The hydro alcoholic extract and ethyl acetate fraction demonstated significant free radical scavenging activity, indicating a high antioxidant potential, while the n-hexane and aqueous fraction is particularly enriched with potent antioxidant compounds. The results collectively validate the ethnomedicial relevance of ehretia laevis and provide a basis for its further pharmacological exploration.
Keywords: EHRETIA LAEVIS, Antioxidant Potential, DPPH (2,2-Diphenyl-1-Picrylhydrazyl) and Nitic oxide Scavenging assays.

Cite This Article:

Please cite this article in press Vinjavarapu L. Anusha*et al., In Vitro Antioxidant Evaluation Of Flowres Of Ehretia Laevis Roxb By Dpph Radical Scavenging Assay And Niric Oxide Radical Scavenging Assay., Indo Am. J. P. Sci, 2025; 12(07).

Number of Downloads : 10

References:

1.Rahul Deshpande comparative evaluation of antimicrobial properties of Different Extracts of “Ehretia laevis” against salivary microflora. Research journal of pharmaceutical, biological and research journal of pharmaceutical, biological and chemical, 2018, 4(12), 235-245.
2. Rahman, K.studies on free radicals, antioxidants, and co-factors. Clin. Interv. Aging 2007, 2, 219-236.
3. Kataria, A.K.;Kataria, N.Evaluation of oxidative stress in sheep affected with peste des petits ruminants. J., 2007, 173, 502-511.
4. Gandhimathi, G.;Bai, G.V.S. In vitro antioxidant activity of randia dumetorum lam leaf extract. Int, J. Herb Med., 2013, 1, 107-111.
5. Pandey A, Tripathi S. Concept of standardization, extraction and pre phytochemical screening strategies for herbal drug. J Pharmacogn Phytoche., 2014; 2(5): 115-119
6. Sen, s.; Chakraborty, R.; Sridhar, c.; Reddy, Y.S.R.; DE, B. Free radicals, Antioxidants, disease and phytomedicines: Current status and future prospect. Int. J. pharm. Sci. Rev. Res., 2010,3, 91-100.
7. Mathew, S.; Abraham, E.T. In Vitro antioxidant activity and scavenging effects of cinnamon verum leaf extract assayed by different methodologies. Food Chem. Toxicol., 2006, 44, 198-206.