Volume : 12, Issue : 06, June – 2025
Title:
FORMULATION AND EVALUATION OF AN ANTIMICROBIAL HERBAL SOAP PAPER
Authors :
V.Gnaneshwari*, R. Raveena, R. Akhila, R. Anusha, S. Hanumanthu Nayak, S. Aishwarya
Abstract :
The present study aims to formulate and evaluate an antimicrobial herbal soap using Cassia auriculata and Nelumbo nucifera flower extracts, which are traditionally known for their medicinal and skin-beneficial properties. With the growing interest in herbal and chemical-free personal care products, this research focuses on harnessing the natural antimicrobial and antioxidant properties of these flowers to develop an effective herbal soap. The extracts were prepared and incorporated into a standard soap base using the cold saponification method. The formulated soap was subjected to various physicochemical evaluations, including pH, foam height, hardness, spreadability, and stability. Antimicrobial efficacy was tested against common skin pathogens such as Staphylococcus aureus and Escherichia coli using the agar well diffusion method. The results demonstrated that the herbal soap possessed significant antimicrobial activity, along with acceptable physical properties and stability. This study highlights the potential of Cassia auriculata and Nelumbo nucifera as valuable natural ingredients in the formulation of herbal antimicrobial soaps, offering a safe and effective alternative to synthetic cleansing products.
Keywords: Cassia Auriculata And Nelumbo Nucifera Flower Extracts, Herbal Soap.
Cite This Article:
Please cite this article in press V.Gnaneshwari al., Formulation And Evaluation Of An Antimicrobial Herbal Soap Paper, Indo Am. J. P. Sci, 2025; 12(06).
Number of Downloads : 10
References:
1. Nasrul wathoni, ani haerani, nia yuniarsih. A Review on Herbal CosmeticsIn Indonesia. International journal of applied pharmaceutics, Innovare academic science, ISSN 0975- 7058, 2018; 10: 5.
2. Evariste Fongnzossie Fedoung, Tize Zra, Christine Fernande Nyangono Biyegue Achille Nouga Bissoue, Suzanne Baraye, and Nole Tsabang. Herbal Cosmetics Knowledge of Arab-Choa and Kotoko Ethnic Groups in the Semi-Arid Areas of Far North Cameroon: Ethnobotanical Assessment and Phytochemical Review. Cosmetics, 2018; 5: 31. doi:10.3390/cosmetics5020031.
3. Rostamailis. Perawatan badan, kulit, dan ram but. Jakarta: Rineka Cipta, 2005.
4. Kareru, P. G., Keriko, J. M., Kenji, G. M., Thiong’o, G. T., Gachanja, A.N., & Mukiira, H. N. Antimicrobial activities of skincare preparations from plant extracts. African Journal of Traditional, Complementary and Alternative Medicines, 2010; 7(3).
5. Bandyopadhyay, U., Biswas, K., Sengupta, A., Moitra, P., Dutta, P., Sarkar, D., … & Banerjee, R. K. Clinical studies on the effect of Neem (Azadirachta indica) bark extract on gastric secretion and gastroduodenal ulcer. Life sciences, 2004; 75(24): 2867-2878.
6. Reddy, Y. R. R., Kumari, C. K., Lokanatha, O., Mamatha, S., & Reddy, C. D. Antimicrobial activity of Azadirachta Indica (neem) leaf, barkand seed extracts. Int. J. Res. Phytochem. Pharmacol, 2013; 3(1): 1-4. 2.
7. Afsar, Z., Khanam, S., & Aamir, S. Formulation and comparative evaluation of polyherbal preparations for their disinfectant effects, 2018; 1(1).
8. Joshi, M. G., Kamat, D. V., & Kamat, S. D. Evaluation of herbal handwash formulation, 2008; 7(5): 413-15.
9. Dhanasekaran, M. International research journal of pharmacy, 2016; 7(2): 31-35.
10. Shivanand, P., Nilam, M., & Viral, D. Herbs play an important role in the field of cosmetics. International Journal of Pharm Tech Research, 2010; 2(1): 632-639.
11. Hassanzadeh P, Bahmani M, Mehrabani D. Bacterial resistance to antibiotics in acne vulgaris: An in vitro study. Indian J Dermatol, 2008; 53(3): 122-4. doi: 10.4103/0019- 5154.43213, PMID 19882009.
12. Laily N, Kusumaningtyas RW, Sukarti I, Rini MRDK. The potency of guava Psidium guajava (L.) leaves as a functional immunostimulatory ingredient. Procedia Chem, 2015; 1, 14: 301-7. doi: 10.1016/j.proche.2015.03.042.
13. Zaman SU, Akhtar N. Effect of Turmeric (Curcuma longa Zingiberaceae) Extract Cream on Human Skin Sebum Secretion. TropJ Pharm Res, 2013; 29, 12(5): 665-9. doi: 10.4314
14. Bhalekar MR, Padher S, Ashwini M, Madgulkar R. Evaluation of aqueous extract of Soapnut as surfactant in cosmetics. J Pharmacogn Phytochem, 2017; 6(4): 1318-2.
15. Namiesnik and rabba jczyk, Wilson. Fresh water parameter and specification of physiochemical forms of metal in surface water and sediments. Chemical’s specification and bioavailability 2010-11;22(1):1-22.
16. Fried Man M, Wolf. Chemistry of soap and detergent various types commercial products and their ingredient. Clinical dermatology 1996; 14:7-14.
17. Richards MJ, Edwards JR, Culver DH, Gayness RP. Nosocomial infections in medical intensive care units in the United States. National Nosocomial Infections Surveillance System. Crit. Care Med 1999; 27:887-892.
18. Lucet JC, Rigaud MP, Mentre F, Kassis N, Deblangy C, Andremont A, et al. mi nation before and after different hygiene techniques: a randomized clinical trial. J. Hosp. Infect 2002; 50:276-280.
19. Alamzafar (queens, NY, US 07/25 2002, One Time Use disposable paper soap and method of making: application 200-20098994.
20. Siddons, as. August 20. How is a non-soap bar different from soap? Retrieved from http. //house Stuff work. Com/skin. Care/cleansing/product/non-soap bar 2009.
21. Namiesnik and rabba jczyk, Wilson. Fresh water parameter and specification of physiochemical forms of metal in surface water and sediments. Chemical’s specification and bioavailability 2010-11;22(1):1-22
22. Jogu Chandrudu. Formulation and standardization of Polyherbal Hand Sanitizer. International Journal of Pharmaceutical Research and Life Sciences, 2020, 7(1), 10–13. doi:10.26452/ijprls. v7i1.1181.
23. Friedman, M. and Wolf, R. Chemistry of soaps and detergents: Various types of commercial products and their ingredients. Clinics in Dermatology, 2019, 14(1), 7–13. Doi: 10.1016/0738- 081x (95)00102-l.
24. Kareru, P.G. Antimicrobial activities of skincare preparations from plant extracts. African Journal of Traditional Complementary and Alternative Medicines. 2010, 7(3). doi:10.4314/ajtcam. v7i3.54777.
25. Afsar, Z. and Khanam S. Formulation and evaluation of Poly Herbal Soap and hand sanitizer. International Research Journal of Pharmacy, 2016,7(8), 54–57. doi:10.7897/2230-8407.07896.
26. Borkar A, Borkar R, Deshmukh S. A review on formulation and evaluation of Polyherbal Soap. International Journal of Research Publication and Reviews. 2023, 4(10), 2315–2321. doi:10.55248/gengpi.4.1023.102712.
27. Formulation and evaluation of Multi Herbal Face Wash Gel. International Journal of Biology, Pharmacy and Allied Sciences, 9(5). doi:10.31032/ijbpas/2020/9.5.5027.
28. Antimicrobial activity and phytochemical analysis of some selected plants against clinical pathogens. Tropical Journal of Natural Product Research, 2021, 5(4), 732–738.
29. Surjushe A, Vasani R, Saple D. Aloe Vera: A short review’, Indian Journal of Dermatology, 2008, 53(4), 163. doi:10.4103/0019-5154.44785.
30. Ryu, S. Colonization and infection of the skin by S. Aureus: immune system evasion and the response to cationic antimicrobial peptides. International Journal of Molecular Sciences, 2014, 5(5), 8753–8772. doi:10.3390/ijms15058753.
31. A review on herbal cosmetics used in skin and hair care’ (2023) International Research Journal of Modernization in Engineering Technology and Science. Doi: 10.56726/irjmets37305.
32. Singh, S. and Majumdar, D.K. Antimicrobial activity of fixed oil of Ocimum sanctum – its role in inflammatory diseases. Therapeutic Drug Monitoring, 1999, 21(4), 462. Doi: 10.1097/00007691-199908000-00143.
33. Mafakheri, S. Effect of bio and chemical fertilizers on the essential oil content and constituents of melissa officinalis (lemon balm)’, Journal of Essential Oil-Bearing Plants, 2016,19(5), 1277– 1285. doi:10.1080/0972060x.2014.983995
34. Sridharan, B. Beneficial effect ofcitruslimonpeel aqueous methanol extract on experimentally induced Urolithic Rats’, Pharmaceutical Biology,2015, 54(5), 759–769. doi:10.3109/13880209.2015.1079724.
35. Adebukola Adeyanju, A. (2023) ‘Inhibition of multidrug resistance by polyphenolic phytochemicals of citrus fruits’, Citrus Research – Horticultural and Human Health Aspects [Preprint]. doi:10.5772/intechopen.107903.
36. Rifai, N. The development and prospect of Indonesian palm oil industry and its derivative products’, IOSR Journal of Economics and Finance, 2014, 4(5), 27–39. doi:10.9790/5933- 0452739.
37. Alashek, F., Keshe, M. Alhassan, G. Preparation of glycerol derivatives by entered of glycerol in different chemical organic reactions: A Review’, Results in Chemistry, 100359. doi: 10.1016/j.rechem.2022.100359.
38. Black phosphorus nanosheets-based hydrogel for efficient bacterial inhibition and accelerating wound healing [Preprint]. doi:10.1021/acsami. 4c06075.s001.
39. Ingold, K.U. Vitamin E remains the major lipid-soluble, chain-breaking antioxidant in human plasma even in individuals suffering severe vitamin E deficiency’, Archives of Biochemistry and Biophysics, 1997,259(1), 224–225. doi:10.1016/0003-9861(87)90489-9.
40. Nemes Z, Steinert P., Bricks and mortar of the epidermal barrier’, Experimental & Molecular Medicine, 2009, 1(1),5–19. doi:10.1038/emm.1999.2.
41. Maderson P. Skin: biochemistry and physiology of the skin Lowell A. Goldsmith, ed. Oxford University Press, New York, 1983. in two volumes. XL, 1324, illus. Science, 224(4648), 486– 486. doi:10.1126/science.224.4648.486. a.
42. Wanjari, A.K. Formulation, evaluation and physical properties of herbal green hand wash prepared from methanolic extract of Delonix Regia leaves’, International Journal of Research in Ayurveda and Pharmacy, 2020, 11(3), pp. 58–60. doi:10.7897/2277-4343.110360.
43. Formulation & Evaluation of consumer-friendly poly herbal hand wash. International Journal of Biology Pharmacy and Allied Sciences, 2024 13(4).
44. Dutta, A.K. Bringing up preterm babies—a guide for parents: Krisha Krishnani and Umesh Vaidya (eds)’, The Indian Journal of Paediatrics’, 2014, 81(11), pp. 1281–1281. doi:10.1007/s12098-014-1439-y.
45. Rajarathinam, M. and Kalaichelvan, P.T. Biogenic NANOSILVER as a potential antibacterial and antifungal additive to commercially available dish wash and hand wash for an enhanced antibacterial and antifungal activity against selected pathogenic strains’, IRJP 2013,4(7), 68–75. doi:10.7897/2230-8407.04715.
46. Krishnamoorthy, K. and Senguttuvan, J. (2016) ‘Evaluation of anti-inflammatory and antioxidant properties of crude extract and forskolin from Solena Amplexicaulis Leaf’, Indian Journal of Pharmaceutical Sciences, 78(3). doi:10.4172/pharmaceutical-sciences.1000128.
47. Saikia A.P., Ryakala V.K., Sharma P., Goswami P., Bora U; Ethnobotany of medicinal plants used by Assamese people for various skin ailments and cosmetics. Journal of Ethnopharmacology, June 2006; 106(2): 149-157.
48. Amar Surjushe, Resham Vasani, and D G Saple; A Short Review; Aloe Vera; Indian Journal of Dermatology, February 2008; 53(4): 163-166
49. Proksch E, Brandner JM, Jensen JM, The skini an indispensable barrier. Exp Dermantol, 2008; 17: 1063-72.
50. Maru AD, Lahoti SR. Formulation and evaluation of moisturizing cream containing sunflower wax Int J Pharma sci., 2018; 11: 54-9.
51. Patwardhan B. Vaidya AD, Chorghade M. Ayurveda and natural product drug discovery curr sci., 2004; 86: 789-99.
52. Hsieh M, Chen C. Delivery of pharmaceutical agent to treat acne vulgaris: Current status and perspectives. J Med Biol., 2011.
53. Shweta Patil, Shruti Patil, Sakshi Kadam, Pranita Adate, Namrata Gurav and Yash Kamble. Formulation and Evaluation of Herbal Paper Soap. Vol 14, Issue 08, 2025.
54. Kadam Komal Keshavrao, Pawar Dipali Vijay, Garode Pratiksha Ganesh, Bahekar Geeta Shiv Narayan, Prof. Sachin Munde, Formulation and Evaluation of Herbal Soap Using Leaves, International Journal of Sciences and Innovation Engineering, Volume 2, Issue 6 June-2025.
55. Chandrashekhar K. Sangle, Prof. Poonam P. Khade, Dr. Megha T. Salv Formulation and Evaluation of Multifunctional Herbal Soap Paper Strips from Tulsi, Turmeric, And Neem. Ijariie, Vol-10 Issue-3 2024.