Volume : 12, Issue : 03, March – 2025
Title:
FORMULATION, DEVELOPMENT AND EVALUATION OF ANTI-OXIDANT ACTIVITY OF HERBAL OINTMENT WITH COMMIPHORA MUKUL BARK EXTRACT
Authors :
Anuradha Narwade, Sanjay Toshniwal
Abstract :
Herbal medicine has played a crucial role in healthcare systems across various cultures for centuries, offering natural alternatives to synthetic drugs. With the increasing awareness of the side effects associated with synthetic formulations, there is a growing interest in herbal-based treatments. Commiphora mukul (Guggul), a medicinal plant used extensively in Ayurveda, has been recognized for its potent anti-inflammatory, antioxidant, and wound-healing properties. The present study focuses on the formulation and evaluation of a herbal ointment incorporating Commiphora mukul bark extract, aimed at assessing its potential for wound healing and skin protection.The extract was obtained through Soxhlet and maceration methods using ethanol, methanol, and chloroform as solvents. Phytochemical screening confirmed the presence of bioactive compounds, including flavonoids, tannins, glycosides, and alkaloids, which contribute to its therapeutic properties. The antioxidant potential of the extract was evaluated using the DPPH free radical scavenging assay, which demonstrated significant radical inhibition, confirming its efficacy as a natural antioxidant.
A herbal ointment was formulated using Jathyadi Thailam and petroleum jelly as the base, incorporating Commiphora mukul extract in 5% and 10% concentrations. The physicochemical properties of the ointment, including pH, spreadability, viscosity, and stability, were evaluated according to standard protocols. Results indicated that the formulated ointment exhibited excellent homogeneity, spreadability, and stability over months. The pH was within an acceptable range (5.6-6.2), ensuring compatibility with human skin. The findings suggest that the Commiphora mukul-based ointment possesses significant antioxidant potential and favorable physicochemical properties, making it a promising candidate for skin care and wound healing applications. This study highlights the importance of integrating traditional medicinal plants into modern pharmaceutical formulations, promoting the use of natural alternatives for skin-related ailments. Further in vivo studies and clinical trials are recommended to validate the therapeutic efficacy of the formulated herbal ointment
Keywords: Commiphora mukul, Herbal Ointment, Antioxidant Activity, Phytochemical Screening, DPPH Assay, Wound Healing, Ayurveda, Natural Medicine.
Cite This Article:
Please cite this article in press Anuradha Narwade et al., Formulation, Development And Evaluation Of Anti-Oxidant Activity Of Herbal Ointment With Commiphora Mukul Bark Extract., Indo Am. J. P. Sci, 2025; 12(03).
Number of Downloads : 10
References:
1. Abbas A.B.; Lichtman A.H. (2009),”Ch.2 Innate Immunity”. In saunders(Elsevier). Basic Immunology. Functions and disorders of the immune system (3rd Ed.). ISBN 978-1-4160- 4688.
2. Albertson S, Hummel 3rd RP, Breeden M, Greenhalgh DG. PDGF and FGF reverse the healing impairment in protein-malnourished diabetic mice. Surgery 1993; 114: 368-72.
3. Ahmad, F., Rasheed, Preliminary screening of methanolic extracts of Celastrus paniculatus and Tecomella undulata for analgesic and anti-inflammatory activities. Journal of Ethnopharmacology 42, 193–198(1993).
4. Alvala Ravia, A. Mallikab, Venkatesh Samaa, A. Sajeli Begumb, Rukaiyya S. Khanb, B. Madhava Reddya G. Pulla Reddy College of Pharmacy, Mehdipatnam, Hyderabad-500 028, AP, India, Antiproliferative activity and standardization of Tecomella undulata bark extracton K562 cells, Journal of Ethnopharmacology 137 (2011) 1353– 1359.
5. Balducci L, Alessandri G. Isolation, Expansion, and Immortalization of Human Adipose- Derived Mesenchymal Stromal Cells from Biopsies and Liposuction Specimens. Methods Mol Biol 2016; 1416: 259-74. doi: 10.1007/978-1-4939- 3584-0_15. 18.
6. Chandra Shekhar Singh, Shailesh Gupta, Alok Pal jain , SRK University,NH- 12 Hoshangabad Road, Misrod, Bhopal, India Millennium college of Pharmacy,Bhopal, India.2019.JETIR June 2019, Volume 6, Issue 6.
7. Dr Puneshwar Keshari, Dr. Pradeep and Suchitra N Prabhu, PG Scholar, Department of Dravya Guna, SDM College of Ayurveda and Teaching Hospital, Hassan, Karnataka, India. pharmacognostical and chromatographic evaluation of market sample of rhododendron arboreum stem bark as a source plant for rohitaka in nepal, Journal of Pharmacognosy and Phytochemistry 2017; 6(5): 296-306.
8. Durmus A, Han MC, Yaman I. Comperative evaluation of collagenase and silver sulfadiazine on burned wound healing in rats. Firat Universitesi Saglik Bilimleri Veteriner Dergisi 2009; 23: 135-9. 17.
9. Eppley BL, Woodell JE, Higgins J. Platelet quantification and growth factor analysis from platelet-rich plasma: implications for wound healing. Plast Reconstr Surg 2004; 114: 1502- 8. doi: 10.1097/01.PRS.0000138251.07040.51.
10. Essentials of pathophysiology: Concepts of altered health states. Hagerstown, MD: Lippincott Williams &Wilkins. P. 270. ISBN 0-7817-7087-4, Porth, Carol (2007).
11. Henrik H. Steenfos ;Growth Factors And Wound Healing ; From The Department of Plastic Surgery, Herlev University Hospital, Herlev,Denmark; January 25,1993.
12. J M McHugh et al. Pain: Neuroanatomy, Chemical mediators, and clinical imlication; AACN Clin Issues ;2000 may.