Volume : 12, Issue : 11, November – 2025
Title:
SYNTHESIS, ANALYSIS AND ANTI BACTERIAL ACTIVITY EVALUATION OF DI SUBSTITUTED BENZODIAZEPINE DERIVATIVES
Authors :
Marka Shalini, Dr. L. Harikiran
Abstract :
The present study focuses on the synthesis, characterization, and antibacterial evaluation of novel di-substituted benzodiazepine derivatives. Benzodiazepines, a class of heterocyclic compounds containing a fused benzene and diazepine ring, possess a wide range of biological activities, including antimicrobial, anti-inflammatory, and central nervous system effects. In this research, a series of di-substituted benzodiazepine derivatives were synthesized through a condensation reaction between o-phenylenediamine and various substituted ketones or aldehydes under controlled conditions. The synthesized compounds were purified and characterized using Fourier-transform infrared spectroscopy (FT-IR), proton nuclear magnetic resonance (¹H-NMR), carbon-13 nuclear magnetic resonance (¹³C-NMR), and Mass Spectrometry to confirm their structures and purity.
The antibacterial potential of the synthesized derivatives was evaluated against selected Gram-positive (Staphylococcus aureus, Bacillus subtilis) and Gram-negative (Escherichia coli, Pseudomonas aeruginosa) bacterial strains using the agar well diffusion method. The results indicated that several derivatives displayed moderate to significant antibacterial activity, comparable to standard antibiotics such as ciprofloxacin. The observed differences in activity suggest that the nature and position of substituents on the benzodiazepine ring significantly influence biological efficacy, indicating a clear structure–activity relationship (SAR).
Keywords: Benzodiazepine derivatives; Synthesis; Spectral analysis; Antibacterial activity; Structure–activity relationship (SAR); Heterocyclic compounds; Drug design
Cite This Article:
Please cite this article in press Marka Shalini et al., Synthesis, Analysis And Anti Bacterial Activity Evaluation Of Di Substituted Benzodiazepine Derivatives, Indo Am. J. P. Sci, 2025; 12(11).
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