Volume : 12, Issue : 10, October – 2025
Title:
FORMULATION AND EVALUATION OF ANTIFUNGAL LOTION USING TEA TREE OIL AND LEMONGRASS EXTRACT
Authors :
Neha A. Rodge*, Prof. Shubham Tikait, Dr. Swati P. Deshmukh
Abstract :
The present study focuses on the formulation and evaluation of a herbal antifungal lotion incorporating Tea Tree Oil (Melaleuca alternifolia) and Lemongrass Oil (Cymbopogon citratus), both known for their potent antimicrobial and antifungal activities. The objective was to develop a safe, effective, and skin-friendly topical preparation to combat superficial fungal infections. An oil-in-water (O/W) emulsion type lotion was prepared using standard emulsification techniques, with varying concentrations of the essential oils to identify the optimal formulation. The developed formulations were subjected to physicochemical evaluations such as ph, viscosity, spreadability, homogeneity, and stability studies, along with antifungal activity testing against Candida albicans and Aspergillus niger using the agar well diffusion method. Among the batches (F1–F3), formulation F2 exhibited ideal physicochemical properties, a skin-compatible ph (6–7), superior spreadability, and the highest antifungal efficacy. The results confirm the synergistic antifungal potential of Tea Tree and Lemongrass oils, offering a promising natural alternative to synthetic antifungal agents with fewer side effects. The study concludes that the optimized formulation can serve as an effective, stable, and eco-friendly topical therapy for the management of superficial fungal infections.
Keywords: Antifungal lotion, Tea Tree Oil, Lemongrass Oil, Herbal formulation, Oil-in-water emulsion, Topical therapy.
Cite This Article:
Please cite this article in press Neha Ashok Rodge et al., Formulation And Evaluation Of Antifungal Lotion Using Tea Tree Oil And Lemongrass Extract, Indo Am. J. P. Sci, 2025; 12(10).
REFERENCES:
1 Carson CF, Hammer KA, Riley TV. Melaleuca alternifolia (Tea Tree) Oil: A Review of Antimicrobial and Other Medicinal Properties. Clinical Microbiology Reviews. 2006;19(1):50–62. [PMC1360273]
2 Hammer KA, Carson CF, Riley TV. Antifungal effects of Melaleuca alternifolia (tea tree) oil and its components against Candida spp. Journal of Antimicrobial Chemotherapy. 2004;53(6):1081–1085.
3 Aldawsari HM, et al. Design and formulation of a topical hydrogel integrating lemongrass-loaded nanosponges with enhanced antifungal effect. International Journal of Nanomedicine. 2015;10:893–902.
4 Wróblewska M, et al. The influence of tea tree oil on antifungal activity and pharmaceutical characteristics of Pluronic F-127 gel formulations with ketoconazole. Scientific Reports. 2021;11(1):22009.
5 Ali, B., Al-Wabel, N. A., Shams, S., Ahamad, A., Khan, S. A., & Anwar, F. (2015). Essential oils used in aromatherapy: A systemic review. Asian Pacific Journal of Tropical Biomedicine, 5(8), 601–611.
6 Carson, C. F., Hammer, K. A., & Riley, T. V. (2006). Melaleuca alternifolia (Tea Tree) Oil: A Review of Antimicrobial and Other Medicinal Properties. Clinical Microbiology Reviews, 19(1), 50–62.
7 Majewska, E., & Góral, T. (2019). Lemongrass (Cymbopogon citratus) Essential Oil: Extraction and Characterization. Polish Journal of Environmental Studies, 28(5), 2591–2597.
8 Lachman, L., Lieberman, H. A., & Kanig, J. L. (2013). The Theory and Practice of Industrial Pharmacy (4th ed.). CBS Publishers & Distributors Pvt. Ltd.
9 Aulton, M. E., & Taylor, K. M. G. (2017). Aulton’s Pharmaceutics: The Design and Manufacture of Medicines (5th ed.). Elsevier Health Sciences
10 Jain, S., Thakur, A., & Gupta, M. (2021). Development of oil-in-water herbal emulsion using natural essential oils. Journal of Drug Delivery and Therapeutics, 11(4), 15–21.
11 Kumar, A., Singh, R., & Yadav, D. (2019). Formulation and evaluation of topical herbal antifungal lotion. Research Journal of Pharmacy and Technology (RJPT), 12(9), 4301–4306.
12 Nair, R., Patel, B., & Shah, P. (2020). Formulation and evaluation of herbal lotion containing essential oils for antimicrobial activity. International Journal of Pharmaceutical Sciences and Research (IJPSR), 11(8), 3954–3961.
13 Sharma, P., Kaur, G., & Tiwari, N. (2022). Comparative antifungal activity of herbal lotions containing Tea Tree Oil and Lemongrass Oil. International Journal of Pharmaceutical Sciences Review and Research (IJPSRR), 73(1), 1–7.
14 Patel, R., Chaudhary, S., & Mehta, T. (2021). Effect of emulsifying agents on the stability and pH of lotion formulations. Asian Journal of Pharmaceutical and Clinical Research (AJPCR), 14(3), 122–127.




