Volume : 12, Issue : 10, October – 2025
Title:
EVALUATION OF ANTI-DIURETIC ACTIVITY USING A COMBINATION OF ETHANOLIC ROOT EXTRACTS OF ECLIPTA ALBA AND OCIMUM SANCTUM
Authors :
Dr. Mehnoor Farheen*, Shamma Sultana, Syeda Qadar Unnisa, Khadija Ameen, Dr. Abdul Aziz Shahid, Mahin Fatima
Abstract :
The present study aimed to investigate the antidiuetic potential of ethanolic root extracts of Eclipta alba(EA) and Ocimum sanctum(OS) , two plants used in ayurvedic formulations. The extracts were obtained using the maceration technique with ethanol as the solvent. Primary phytochemicals screening revealed the presence of various bioactive compounds, including alkaloids, tannins, flavonoids, saponins, glycosides,carbohydrates,coumarins and polyphenols. The investigation utilized in vivo and in vitro methodologies to assess antidiuretic activity,employing Albino Wistar rats as the experimental model. The animals were randomly assigned to nine groups, including a normal control , a standard group administered vasopressin (0.13 mg/rat), and multiple test groups treated with either individual or combined doses of EA and OS. Urine volume, electrolytes excretion (sodium, potassium and chloride), and biochemical markers were measured to assess antidiuretic modulation. The fusion of EA and OS at 400 mg/kg is important in reducing urine output, showing comparable efficacy to vasopressin. These findings suggest that the extracts possess strong antidiuretic properties, likely due to their phytochemical fingerprinting. The results support the herbal use of these herbs and suggest their potential application in managing disorders related to fluid imbalance. The EA & OS at 400 mg/kg significantly reduce urine output compared to the control and furosemide groups (p < 0.01).Keywords: Eclipta alba, Ocimum sanctum, Antidiuretic activity, Ethanolic extract, Furosemide, Vasopressin, Wistar rats, Phytoconstituents.
Cite This Article:
Please cite this article in press Mehnoor Farheen et al., Evaluation Of Anti-Diuretic Activity Using A Combination Of Ethanolic Root Extracts Of Eclipta Alba And Ocimum Sanctum, Indo Am. J. P. Sci, 2025; 12(10).
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