Volume : 12, Issue : 10, October – 2025
Title:
RP-HPLC ANALYTICAL METHOD DEVELOPMENT AND VALIDATION FOR SIMULTANEOUS ESTIMATION OF CIPROFLOXACIN AND TINIDAZOLE AND ITS PHARMACEUTICAL DOSAGE FORMS
Authors :
G. Sindhu a* , Dr. G. Sai kiran, dr. D. Venkata Ramana.
Abstract :
A simple, accurate, and robust Reverse Phase High-Performance Liquid Chromatographic (RP-HPLC) method was developed and validated for the simultaneous estimation of Ciprofloxacin and Tinidazole in combined pharmaceutical dosage forms. The chromatographic separation was achieved on a Develosil C18 column (4.6 mm × 250 mm, 5 µm particle size) using a mobile phase consisting of Acetonitrile and Acetate buffer (pH 4.3) in the ratio of 35:65% v/v, at a flow rate of 1.0 ml/min. The detection was carried out at a wavelength of 238 nm using a Waters Alliance 2695 HPLC system equipped with a PDA Detector (996 model). The injection volume was 20 µl, and the run time was 6 minutes under ambient temperature conditions. The method was validated according to ICH Q2(R1) guidelines for various parameters including specificity, linearity, accuracy, precision, LOD, LOQ, robustness, and system suitability. The results demonstrated high resolution and selectivity between Ciprofloxacin and Tinidazole with no interference from excipients. The method showed excellent linearity and recovery within acceptable limits.
Keywords: RP-HPLC, Ciprofloxacin, Tinidazole, Develosil C18 column, linearity, accuracy.
Cite This Article:
Please cite this article in press G. Sindhu et al., Rp-Hplc Analytical Method Development And Validation For Simultaneous Estimation Of Ciprofloxacin And Tinidazole And Its Pharmaceutical Dosage Forms , Indo Am. J. P. Sci, 2025; 12(10).
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