Volume : 12, Issue : 10, October – 2025

Title:

PHARMACOLOGICAL SCREENING OF ANTIDIABETIC ACTIVITY OF ETHANOLIC EXTRACTS OF BUTEA MONOSPERMA ON STREPTOZOCIN INDUCED RATS

Authors :

Muskaan*, Mrs. Geetha Vani, Dr. D. Venkata Ramana

Abstract :

Diabetes mellitus is a most common endocrine disorder, affecting more than 300 million people worldwide. For these therapies developed along the principles of allopathic are often limited in efficacy, Carry the risk of adverse effects, and are often too costly, especially for the developing world. In order to identify complementary or alternative approaches to existing medications, we studied the anti-diabetic potential of leaves of Butea Monosperma. The acute oral toxicity studies of the extracts revealed no toxic effects up to the levels of 2000mg/kg b.wt. The aqueous and alcoholic extracts of 20 and 30mg/kg body weight of Butea Monosperma was screened for the presence of hypoglycemic and antidiabetic activity. In this study diabetes was induced by a single IP dose Alloxan monohydrate in 72hrs fasted rats. The FBGL was carried on 7th, 14th and 21st day and OGTT was measured on 8th, 15th and 22nd day. Glibenclamide was taken as the standard and the results are quite comparable with it. The studies were indicated that the leaves of Butea Monosperma are effective in regeneration of insulin secreting β-cells and thus possess antidiabetic activity. The aqueous and alcoholic extracts showed significant effect in decreasing the Fasting blood Glucose level and oral glucose tolerance test of rats and it’s also showed good hypoglycemic activity in normal glycemic rats. The preliminary phytochemical analysis of the extracts of Butea Monosperma revealed the presence of Alkaloids, Tannins, Anthraquinones, Flavonoids, Saponins, Triterpenes, Sterols, Coumarin as the possible biologically active principles.
Keywords: Butea Monosperma, Alloxan monohydrate, Glibenclamide, FBGL and OGTT.

Keywords: RP-HPLC, Ciprofloxacin, Tinidazole, Develosil C18 column, linearity, accuracy.

Cite This Article:

Please cite this article in press Muskaan et al., Pharmacological Screening Of Antidiabetic Activity Of Ethanolic Extracts Of Butea Monosperma On Streptozocin Induced Rats , Indo Am. J. P. Sci, 2025; 12(10).

REFERENCES:

1. Consultation, W. H. O. Definition, diagnosis and classification of diabetes mellitus and its complications. Vol. 1. Part, 1999.
2. Alberti, Kurt George Matthew Mayer, and PZ ft Zimmet. “Definition, diagnosis and classification of diabetes mellitus and its complications. Part 1: diagnosis and classification of diabetes mellitus. Provisional report of a WHO consultation.” Diabetic medicine 15.7 (1998): 539-553.
3. Gress, Todd W., et al. “Hypertension and antihypertensive therapy as risk factors for type 2 diabetes mellitus.” New England Journal of Medicine 342.13 (2000): 905-912.
4. Ahmed, Awad M. “History of diabetes mellitus.” Saudi medical journal 23.4 (2002): 373-378.
5. Zimmet, Paul Z., Daniel J. McCarty, and Maximilian P. de Courten. “The global epidemiology of non-insulin-dependent diabetes mellitus and the metabolic syndrome.” Journal of Diabetes and its Complications 11.2 (1997): 60-68.
6. American Diabetes Association. “Type 2 diabetes in children and adolescents.”Pediatrics 105.3 (2000): 671-680.
7. Saltiel, Alan R., and C. Ronald Kahn. “Insulin signalling and the regulation of glucose and lipid metabolism.” Nature 414.6865 (2001): 799-806.
8. Browning, Jeffrey D., and Jay D. Horton. “Molecular mediators of hepatic steatosis and liver injury.” Journal of Clinical Investigation 114.2 (2004): 147-152.
9. Aronoff, Stephen L., et al. “Glucose metabolism and regulation: beyond insulin and glucagon.” Diabetes Spectrum 17.3 (2004): 183-190.
10. Ross, E. J., and D. C. Linch. “Cushing’s syndrome—killing disease: discriminatory value of signs and symptoms aiding early diagnosis.” The Lancet320.8299 (1982): 646-649.
11. Levin, Marvin E., Vincenza C. Boisseau, and Louis V. Avioli. “Effects of diabetes mellitus on bone mass in juvenile and adult-onset diabetes.” New England Journal of Medicine 294.5 (1976): 241-245.
12. Sherwin, Robert, and Philip Felig. “Pathophysiology of diabetes mellitus.” The Medical clinics of North America 62.4 (1978): 695-711.
13. Faber, O. K., and C. Binder. “C-peptide response to glucagon: a test for the residual β-cell function in diabetes mellitus.” Diabetes 26.7 (1977): 605-610.
14. Ioannidis, Ioannis. “Pathophysiology of Type 1 diabetes.” Diabetes in Clinical Practice: Questions and Answers from Case Studies 31 (2007): 23.
15. Scheen, A. J. “Pathophysiology of type 2 diabetes.” Acta Clinica Belgica 58.6 (2003): 335-341.
16. Vambergue, A., et al. “[Pathophysiology of gestational diabetes].” Journal de gynecologie, obstetrique et biologie de la reproduction 31.6 Suppl (2002): 4S3-4S10.
17. Inzucchi, Silvio E. “Diagnosis of diabetes.” New England Journal of Medicine367.6 (2012): 542-550.
18. Turner, R. C., et al. “Intensive blood-glucose control with sulphonylureas or insulin compared with conventional treatment and risk of complications in patients with type 2 diabetes (UKPDS 33).” lancet 352.9131 (1998): 837-853.
19. Brownlee, M., and A. Cerami. “The biochemistry of the complications of diabetes mellitus.” Annual review of biochemistry 50.1 (1981): 385-432.
20. Pari, L., and G. Saravanan. “Antidiabetic effect of Cogent db, a herbal drug in alloxan-induced diabetes mellitus.” Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology 131.1 (2002): 19-25.
21. Day, Caroline. “Traditional plant treatments for diabetes mellitus: pharmaceutical foods.” British Journal of Nutrition 80.01 (1998): 5-6.
22. Smith, George Davey, et al. “Genetic epidemiology and public health: hope, hype, and future prospects.” The Lancet 366.9495 (2005): 1484-1498.
23. Ganesh G. Dhakad, Deepak S. Mohale, A.V. Chandewar. Evaluation of Anti-Diabetic Activity of Galinosa parviflora in Diabetic Rats. Res. J. Pharmacology and Pharmacodynamics.2024;16(3):143-152.
24. Beyene Derej, Aschalew Nardos, Jemal Abdela, Lidet Terefe, Melese Arega, Terfo Mikre Yilma, Tilahun Tesfaye. Antidiabetic Activities of 80% Methanol Extract and Solvent Fractions of Verbascum Sinaiticum Benth (Scrophulariaceae) Leaves in Mice. Journal of Experimental Pharmacology 2023:15 423–436.
25. Kolajo Adedamola Akinyede, Habeebat Adekilekun Oyewusi, Gail Denise Hughes, Okobi Eko Ekpo, and Oluwafemi Omoniyi Oguntibeju. In Vitro Evaluation of the Anti-Diabetic Potential of Aqueous Acetone Helichrysum petiolare Extract (AAHPE) with Molecular Docking Relevance in Diabetes Mellitus. Molecules 2022, 27, 155.
26. Nithiyaa Perumal, Meenakshii Nallappan, Shamarina Shohaimi, Nur Kartinee Kassim, Thiam Tsui Tee, Yew Hoong Cheah. Synergistic antidiabetic activity of Taraxacum officinale (L.) Weber ex F.H. Wigg and Momordica charantia L. polyherbal combination. Biomedicine & Pharmacotherapy 145 (2022) 112401.
27. Yuting Zhu , Hongwei Gao, Shanhao Han, Jianhui Li, Qiqin Wen, Bo Dong. Antidiabetic activity and metabolite profiles of ascidian Halocynthia roretzi. Journal of Functional Foods 93 (2022) 105095.
28. Aligwekwe A. Ugochukwu and Idaguko C. Anna, Phytochemical Evaluation and Anti-Diabetic Effects of Ethanolic Leaf Extract of Petersianthus macrocarpus on Streptozotocin-Induced Diabetic Rats. JAMMR, 33(3): 39-47, 2021.
29. Oluyemisi Omotayo Omonije, Abubakar Ndaman Saidu and Hadiza Lami Muhammad. Anti-diabetic activities of Chromolaena odorata methanol root extract and its attenuation effect on diabetic induced hepatorenal impairments in rats. Clinical Phytoscience (2019) 5:23.
30. Lia Ardiana, Rani Sauriasari, Berna Elya. Antidiabetic Activity Studies of White Tea (Camellia sinensis (L.) O. Kuntze) Ethanolic Extracts in Streptozotocin-nicotinamide Induced Diabetic Rats. Pharmacogn J. 2018; 10(1):186-189.
31. Hanae Naceiri Mrabti, Nidal Jaradat, Ismail Fichtali, Wessal Ouedrhiri, Shehdeh Jodeh, Samar Ayesh, Yahia Cherrah and My El Abbes Faouzi. Separation, Identification, and Antidiabetic Activity of Catechin Isolated from Arbutus unedo L. Plant Roots. 12 April 2018.
32. Ram Niwas Jangir, Gyan Chand Jain. Evaluation of Antidiabetic Activity of Hydroalcoholic Extract of Cassia fistula Linn. pod in Streptozotocin-Induced Diabetic Rats. Pharmacogn J. 2017; 9(5): 599-606.
33. Ameebahen B. Patel ,Dharmeshkumar D. Prajapati, Yogesh Patel. Antidiabetic activity of Moringa oleifera Lam. Algerian Journal of Natural Products 5:2 (2017) 446-453.
34. Md. Uzzal Haque, Samiron Sana. Antidiabetic activity of plants, fruits and vegetables: a review. Int J Biol Med Res.2016;7(1):5452-5458.
35. Juan Wang, Lirong Teng, Yange Liu, Wenji Hu, Wenqi Chen, Xi Hu, Yingwu Wang, and Di Wang. Studies on the Antidiabetic and Antinephritic Activities of Paecilomyces hepialid Water Extract in Diet-Streptozotocin-Induced Diabetic Sprague Dawley Rats. 1 February 2016.
36. Dr. D. Radhika and R. Priya. Assessment Of Anti-Diabetic Activity Of Some Selected Seaweeds. ejbps, 2015, Volume 2, Issue 6, 151-154.
37. Dyah Ratna Wulan, Edi Priyo Utomo,and Chanif Mahdi. Antidiabetic Activity of Ruellia tuberosa L., Role of 𝛼-Amylase Inhibitor: In Silico, In Vitro, and In Vivo Approaches. 28 September 2015.
38. Khalid Ghazanfar, Bashir A. Ganai, Seema Akbar, Khan Mubashir, Showkat Ahmad Dar, Mohammad Younis Dar, and Mudasir A. Tantry. Antidiabetic Activity of Artemisia amygdalina Decne in Streptozotocin Induced Diabetic Rats. 21 May 2014.
39. Abdul Vahab A and Jyoti Harindran. Anti-diabetic Activity of Terminalia catappa Bark Extracts in Alloxan Induced Diabetic Rats. 16/05/2014.
40. Taofik O. Sunmonu and Anthony J. Afolayan. Evaluation of Antidiabetic Activity and Associated Toxicity of Artemisia afra Aqueous Extract in Wistar Rats. 20 May 2013.
41. Anil Kamboj, Sunil Kumar and Vipin Kumar. Evaluation of Antidiabetic Activity of Hydroalcoholic Extract of Cestrum nocturnum Leaves in Streptozotocin-Induced Diabetic Rats. 18 August 2013.
42. Jude E Okokon, Bassey S Antia and John A Udobang. Antidiabetic activities of ethanolic extract and fraction of Anthocleista djalonensis. Asian Pac J Trop Biomed. 2012 Jun; 2(6): 461–464.
43. Himanshu Misra, Manish Soni, Narendra Silawat, Darshana Mehta, B. K. Mehta, and D. C. Jain. Antidiabetic activity of medium-polar extract from the leaves of Stevia rebaudiana Bert. (Bertoni) on alloxan-induced diabetic rats. J Pharm Bioallied Sci. 2011 Apr-Jun; 3(2): 242–248.