Volume : 12, Issue : 10, October – 2025
Title:
EVALUATION OF ANTIBACTERIAL ACTIVITY OF METHANOLIC EXTRACTS OF DATURA STRAMONIUM ON SELECTED BACTERIA
Authors :
Puja Mydampally*, Mr. Arif, Dr. D. Venkata ramana.
Abstract :
This study was carried out with an objective to investigate the antibacterial potentials of leaves of Datura stramonium. The aim of the study is to assess the antimicrobial activity and to determine the zone of inhibition of extracts on some bacterial and fungal strains. In the present study, the microbial activity of methanolic extracts of leaves of Datura stramonium. (an ethnomedicinal plant) was evaluated for potential antimicrobial activity against medically important bacterial and fungal strains. The antimicrobial activity was determined in the extracts using agar disc diffusion method. The antibacterial and antifungal activities of extracts (5, 25, 50, 100, 250 μg/ml) of Datura stramonium were tested against two Gram-positive––Staphylococcus aureus, Streptococcus pyogenes; two Gram-negative––Escherichia coli, Pseudomonas aeruginosa human pathogenic bacteria; and three fungal strains––Aspergillus niger, Aspergillus clavatus, Candida albicans. Zone of inhibition of extracts were compared with that of different standards like ampicillin, ciprofloxacin, norfloxacin, and chloramphenicol for antibacterial activity and nystatin and griseofulvin for antifungal activity. The results showed that the remarkable inhibition of the bacterial growth was shown against the tested organisms. The phytochemical analyses of the plants were carried out. The microbial activity of the Datura stramonium was due to the presence of various secondary metabolites. Hence, these plants can be used to discover bioactive natural products that may serve as leads in the development of new pharmaceuticals research activities.
KEYWORDS: Antibacterial activity and Datura stramonium.
Cite This Article:
Please cite this article in press Puja Mydampally et al., Evaluation Of Antibacterial Activity Of Methanolic Extracts Of Datura Stramonium On Selected Bacteria, Indo Am. J. P. Sci, 2025; 12(10).
REFERENCES:
1. B. Nitha, A.B. Remashree and Indira Balachandran. Antibacterial Activity Of Some Selected Indian Medicinal Plants. IJPSR, 2012; Vol. 3(7): 2038-2042.
2. Chaudhary G, Goyal S, Poonia P. Lawsonia inermis Linnaeus: A Phytopharmacological Review. International Journal of Pharmaceutical Sciences and Drug Research 2010; 2(2): 91-98.
3. Goyal BR, Goyal RK, Mehta AA. Phyto-Pharmacognosy of Archyranthes aspera: A Review. Pharmacognosy Reviews 2008; 1:1.
4. Cragg GM, Newman DJ, Sander KM. Natural products in drug discovery and development. Journal of Natual Products 1997; 60:52-60.
5. Dean DA, Burchard KW. Fungal infection in surgical patients. American Journal of Surgery 1996; 171: 374-382.
6. Gonzalez CE, Venzon D, Lee S et al. Risk factors for fungemia in children infected with human immunodeficiency virus: a case control study. Clinical Infectious Diseases 1996; 23: 515-521.
7. VaghasiyaY, Chanda SV Screening of Methanol and Acetone Extracts of Fourteen Indian Medicinal Plants for Antimicrobial Activity. Turkish Journal of Biology 2007; 31:243-248.
8. Soulsby EJ. Resistance to antimicrobials in humans and animals. Britsh Journal of Medicine 2005; 331: 1219-1220.
9. Cowan MM. Plant products as antimicrobial agents. Clinical Microbiology Reviews 1999; 12: 564-82.
10. Udayan PS, Balachandran I: Medicinal Plants of Arya Vaidya Sala Herb Garden. Department of Publication Arya Vaidya Sala, Firt Edition 2009.
11. Tirtha SS, “The Ayurveda Encyclopedia-Natural Secrets to Healing, Prevention and Longevity”, 2007.
12. S. Chaudhari, N. Shaikh, “Gaduchi-the best ayurvedic herb”, The Pharma Innovation Journal, 2(4), 97-102, 2013. International Journal of Scientific Research and Review Volume 7, Issue 12, 2018 ISSN NO: 2279-543X Page No: 593.
13. U. Spandana, S.L. Ali, T. Nirmala, M. Santhi, S.D. SipaiBabu , “A Review on Tinosporacordifolia”, International Journal of Current Pharmaceutical Review and Research, 4(2), 61-68, 2013.
14. KN. Aiyer, M. Kolammal, Pharmacognosy of Ayuevedic Drugs, Series 1. 1st ed Trivendram, The centeral Research institute, 1963.
15. KR. Kirtikar and BD. Basu, Indian Medicinal Plants. Second Edition, International Book Distributers, Dehradun, 2005.
16. Database on Medicinal Plants Used in Ayurveda, Vol. 3, CCRAS, Reprint, New Delhi, 2005.
17. KM. Nadkarni KM, AK. Nadkarni, Indian Materia Medica. Popular Prakashan Private Limited Mumbai, 3, 1976.
18. Kirtikar KR, Basu BD Indian medicinal plant (2nd ed). Bishen Singh Mahendra Pal Singh, Dehra Dun, vol I, 499–502, 1975.
19. Perez C, Paul M, Bazerque P. An Antibiotic assay by the agar well diffusion method. ActaBio Medica Exp 1990; 15: 113-115.
20. Westh H, Zinn CS, Rosdahl VT, Sarisa Study Group. An international multicenter study of antimicrobial consumption and resistance in S. aureus isolates from 15 hospitals in 14 countries. Microbial Drug Resistance. 2004. 10:169 -176.
21. Shariff ZU. Modern Herbal Therapy for Common Ailments. Nature Pharamcy Series. Spectrum Books Ltd., Ibadan, Nigeria in Association with Safari Books (Export) Ltd. UK, Vol.1,2001: 9-84.
22. Parekh J and Chanda S Antibacterial and phytochemical studies on twelve species of Indian medicinal plants. African Journal of Biomedical Research 2007; 10: 175 – 181.
23. Srinivasan D, Nathan S, Suresh T, Perumalaswamy O. Antimicrobial activity of certain Indian Medicinal Plants used in folkloric medicine. Journal of Ethnopharmocology 2001; 74: 217-220.
24. Mayers D.L., Lerner S.A., Ouelette M. vol. 2. Springer Dordrecht Heidelberg; London: 2009. (Antimicrobial Drug Resistance C: Clinical and Epidemiological Aspects). pp. 681–1347.
25. Jorgensen J.H., Ferraro M.J. Antimicrobial susceptibility testing: a review of general principles and contemporary practices. Clin. Infect. Dis. 2009;49:1749–1755.
26. Caron F. Antimicrobial susceptibility testing : a four facets tool for the clinician. J. Des. Anti-Infect. 2012;14 186-174.
27. Nijs A., Cartuyvels R., Mewis A. Comparison and evaluation of Osiris and Sirscan 2000 antimicrobial susceptibility systems in the clinical microbiology laboratory. J. Clin. Microbiol. 2003;41:3627–3630.
28. Denes É., Hidri N. Synergie et Antagonisme en Antibiothérapie. Antibiotiques. 2009;11:106–115.
29. Sharma S. et al “Topical Drug Delivery System”available from http://www.pharmainfo.net/ accessed on 12/07/2015.
30. Laithy HM. and El shaboury KMF. The development of Cutina Lipogels and gel microemulsion for topical administration of fluconazole. Ame Pharm Sci. PharmSciTech. 2003; 3:10 25.
31. Kshirsagar N A. Drug Delivery Systems. Ind. J. Pharmacol. 2000; 32:S54- S61.
32. Dadwal Meenakshi, emulgel: a novel approach to topical drug delivery International journal of pharma and bio sciences, 4 (2013), 847-856.
33. Rashmi M. Topical gel: A review august vol. 2008; available from http:// www.pharmainfo.com
34. Sharma S. Topical preparations are used for the localized effects at the site of their application by virtue of drug penetration into the underlying layers of skin or mucous membranes. Pharmaceutical reviews 2008; 6:1.
35. Stanos SP. Topical Agents for the Management of Musculoskeletal Pain .J Pain Symptom Manage March 2007; 33.
36. Jain A, Deveda P, Vyas N, Chauhan J et al. Development Of Antifungal Emulsion Based Gel For Topical Fungal Infection(S). IJPRD 2011; 2(12).
37. Bruton L, Keith P, Blumenthal D, Buxton Z. Goodman & Gillman’s Manual of Pharmacology & Therapeutics. Mc Graw’s Hill. 2008. pp.1086-1094.
38. Cecv G. Preclinical characterisation of NSAIDs in ultradeformable carriers or conventional topical gels. International journal of pharmaceutics; 2008.
39. Bhoyar N, Giri T.K, Tripathi D.K, Alexender .A and Ajazuddin: Recent advances in novel drug delivery system through gels: review, J. Pharm. allied health sci, 2012, 2(2), 21-39.
40. Khullar Rachit,Saini S, Seth N and Rana A C:Emulgels: A surrogate approach for topically used hydrophobic drugs,IJPBS, 2011, 1 (3), 117-128.
41. Prajapati Mehulkumar N, Patel M R, Patel K R & Patel N M:Emulgels: a novel approach to topical drug delivery, IJUPBS, 2013, 2(1), 134- 148.
42. Joshi baibhav, Singh Gurpreet, Rana A C, Saini Seema & Singla Vikas: Emulgel: A comprehensive review on recent advancement on topical drug delivery, IRJP, 2011, 2(11).
43. Gaur PK, Mishra S, Purohit S, Dave K. Transdermal Drug Delivery System: A Review. AJPCR 2009; 2: 14-20.
44. Subranayam N, Ghosal SK, Moulik SP. Enhanced In-Vitro Percutaneous Absorption & In-Vivo Anti-Inflammatory Effect of a Selective Cyclooxygenase Inhibitor Using Microemulsion. Drug Dev. & IndustrialPharm., 2005.
45. Pathan, I.B.; Setty, C.M. Chemical penetration enhancers for transdermal drug delivery systems. Trop J Pharm Res. April 2009; 8:173-179.
46. Curr AEB. Transdermal Drug Delivery: Penetration Enhancement Techniques Heather. Drug Deliv, 5(2):23-33, (2005).
47. Rutrer N, Drug absorption through the skin: a mixed blessing. Arch Dis Child, 62:220- 221, (1987).
48. Zhang XL, Zhao R, Qian W. Preparation of an emulgel for treatment of aphthous ulcer on the basis of carbomers. Chin Pharm J, 30:417-418, (1995).
49. Swarbrick, J. Encyclopedia of pharmaceutical technology, 3rd ed., 1551.
50. WB Saunders Co. Philadelphia, 1970, 55-60.
51. Gupta GD, Gound RS. Release rate of nimesulide from different gellants. Indian J Pharm Sci, 61(1): 229-23, (1999).
52. Chaudhari P, Ajab A, Malpure P, Kolsure P, Sanap D, Development and in-vitro evaluation of thermo reversible nasal gel formulations of Rizatriptan benzoate, Indian J. Pharm. Edu. Res., 2009; 43: 55-62.
53. Patel RP, Patel G, Baria A. Formulation and evaluation of transdermal patch of aceclofenac, Int J Drug Del, 1(3): 41 – 51, (2009).
54. Sanjay Jain, B. D., Padsalg, A., Patel, K., & Mokale, V. (2007). Formulation, development and evaluation of Fluconazole gel in various polymer bases, Asi. J. Pharm., 1, 63-68.
55. Masmoudi, H., Piccerelle, P., Le Dreau, Y., & Kister, J. (2006). A rheological method to evaluate the physical stability of highly viscous pharmaceutical oil-in-water emulsions, Pharm. Res., 23 (8), 1937–1947.
56. Jones DB, Woolfson AD, Brown AF. Textural, viscoelastic and mucoadhesive properties of pharmaceutical gels composed of cellulose polymers. Int J Pharm, 151:223–33, (1997).
57. Alookar N.H, Kulkarni A.S, Ingale D.J and Patil R.A:Microsponge as innovative drug delivery system, IJPSN, 2012, 5(1), 1597-1606.
58. M. Vettor, S. Bourgeois, H. Fessi, J. Pelletier, P. Perugini, F. Pavanetto and M. A. Bolzinger: Skin absorption studies of octylmethoxycinnamateloaded poly (D, L-lactide).
59. B.A. Kerwin, “Polysorbates 20 and 80 used in the formulation of protein biotherapeutics: structure and degradation pathways”, J. Pharm. Sci. 97 (2008) 2924–2935.
60. Fuad Al-Rimawi, Mahmood Sbeih, Mousa Amayreh, Belal Rahhal and Samer Mudalal. Evaluation of the antibacterial and antifungal properties of oleuropein, olea Europea leaf extract, and thymus vulgaris oil. Al-Rimawi et al. BMC Complementary Medicine and Therapies (2024) 24:297.
61. Anna Andersone, Sarmite Janceva, Liga Laubert, Anna Ramata-Stunda, Vizma Nikolajeva, Natalija Zaharova, Gints Rieksts, Galina Telysheva. Anti-Inflammatory, Anti-Bacterial, and Anti-Fungal Activity of Oligomeric Proanthocyanidins and Extracts Obtained from Lignocellulosic Agricultural Waste. Molecules 2023 Jan 15;28(2):863 doi: 10.3390/molecules28020863.
62. Amin Mahmood Thawabteh, Zain Swaileh, Marwa Ammar, Weam Jaghama, Mai Yousef 2, Rafik Karaman, Sabino A Bufo, Laura Scrano. Antifungal and Antibacterial Activities of Isolated Marine Compounds. Toxins (Basel) 2023 Jan 18;15(2):93. doi: 10.3390/toxins15020093.
63. Kyu-Shik Lee, Eun-Young Yun, and Tae-Won Goo. Evaluation of Antimicrobial Activity in the Extract of Defatted Hermetia illucens Fed Organic Waste Feed Containing Fermented Effective Microorganisms. Animals 2022, 12, 680.
64. Yidnekachew Girma and Tamene Milkessa Jiru. Evaluation of Antimicrobial Activity of Conyza bonariensis Leaf Extracts against Clinically Isolated Fungi Causing Superficial Infection. Hindawi Journal of Chemistry Volume 2021, Article ID 6367449, 8 pages.
65. Ambaye Kenubih Eyerusalem Belay Kumneger Lemma. Evaluation of the Antimicrobial Activity of Leaf Extracts of Acokanthera schimperi against Various Disease-Causing Bacteria. Journal of Experimental Pharmacology 2021:13 889–899.
66. Jayanthi Chenniappan, A.Sankaranarayanan, S.Arjunan. Evaluation of Antimicrobial Activity of Cissus quadrangularis L. stem extracts against Avian Pathogens and Determination of its Bioactive Constituents using GC-MS. Journal of Scientific Research, Volume 64, Issue 1, 2020.
67. Angelin Jebamalar Jayaraj, Jothi Uchimahali, Thiyagarajan Gnanasundaram, Sivakumar Thirumal. Evaluation Of Antimicrobial Activity And Phytochemicals Analysis Of Whole Plant Extract Of Vinca Rosea. Asian J Pharm Clin Res, Vol 12, Issue 8, 2019, 132-136.
68. Supria Saha, Zohorul Islam, Sadequl Islam, Md. Shahadat Hossain and S. M. Shahinul Islam. Evaluation of antimicrobial activity of wheat (Triticum aestivum L.) against four bacterial strains. SKUAST Journal of Research 20 (1): 58-62 (2018).
69. Hemali Padalia, Tejas Rathod, Sumitra Chanda. Evaluation Of Antimicrobial Potential Of Different Solvent Extracts Of Some Medicinal Plants Of Semi-Arid Region. Asian J Pharm Clin Res, Vol 10, Issue 11, 2017, 295-299.