Volume : 12, Issue : 10, October – 2025
Title:
ANTI-DIABETIC ACTIVITY AND PHYTOCHEMICAL SCREENING OF EXTRACTS OF THE LEAVES OF TINOSPORA CORDIFOLIA ON ALLOXAN-INDUCED DIABETIC MICE
Authors :
Sri Priya *, Arif Mohammad Shaik, Dr. D. Venkata ramana.
Abstract :
In India, the number of people suffering from diabetes is believed to be rising steadily and the current antidiabetic therapies are frequently reported to have adverse side effects. Ethno medicinal plant use has shown promise for the development of cheaper, cost-effective antidiabetic agents with fewer side effects. Te aim of this study was to investigate the antidiabetic activity and mechanism of action of aqueous leaf extract prepared from Tinospora cordifolia. Since this claim has not been investigated scientifically, the aim of this study was to evaluate the antidiabetic effect and phytochemical screening of alloxan-induced diabetic Mice. The leaves of Tinospora cordifolia have been used in traditional health systems to treat diabetes mellitus. However, the antidiabetic activity of this medicinal plant is not scientifically validated and authenticated. The present study aimed to investigate the in vitro and in vivo anti-diabetic activity of flower crude extract and solvent fractions of Tinospora cordifolia. The in vitro α–amylase inhibition of the crude extract and solvent fractions of Tinospora cordifolia. Blood glucose lowering activity of 80% Ethanolic crude extract and solvent fraction was studied in animal models: Hypoglycemic mice model, oral glucose loaded mice model, dose-treated Alloxan -induced diabetic mice model. The effect of the crude extract on diabetic lipid profile was studied.The acute toxicity study of Tinospora cordifolia leaves extract did not show mortality in the animals at the limit dose during the observation period. The result of α–amylase enzyme inhibition activity was found in a dose-dependent manner, the strongest activity was shown by Crude extract fraction (89.60 % inhibition at 1000 μg/mL) compared to the standard acarbose having 97.19% inhibition at 1000 μg/mL. The crude extract of Tinospora cordifolia showed significant blood glucose-lowering effect on hypoglycemic mice and oral glucose loaded mice. In Alloxan-induced diabetic mice model, the crude extract fraction significantly decreased the fasting blood glucose level after 14 days of treatment.The result demonstrated the beneficial biochemical effects of Tinospora cordifolia extract by inhibiting α–amylase improving serum lipid profile levels. The leaves crude extract are effective in lowering blood glucose levels in diabetic and hypoglycemic mice. The claimed traditional use as antidiabetic has scientific ground.
Key words: Diabetes mellitus, Herbal medicine, Tinospora cordifolia, Alloxan, Anti diabetic activity.
Cite This Article:
Please cite this article in press Sri Priya et al., Anti-Diabetic Activity And Phytochemical Screening Of Extracts Of The Leaves Of Tinospora Cordifolia On Alloxan-Induced Diabetic Mice, Indo Am. J. P. Sci, 2025; 12(10).
REFERENCES:
1. Consultation, W. H. O. Definition, diagnosis and classification of diabetes mellitus and its complications. Vol. 1. Part, 1999.
2. Alberti, Kurt George Matthew Mayer, and PZ ft Zimmet. “Definition, diagnosis and classification of diabetes mellitus and its complications. Part 1: diagnosis and classification of diabetes mellitus. Provisional report of a WHO consultation.” Diabetic medicine 15.7 (1998): 539-553.
3. Gress, Todd W., et al. “Hypertension and antihypertensive therapy as risk factors for type 2 diabetes mellitus.” New England Journal of Medicine 342.13 (2000): 905-912.
4. Ahmed, Awad M. “History of diabetes mellitus.” Saudi medical journal 23.4 (2002): 373-378.
5. Zimmet, Paul Z., Daniel J. McCarty, and Maximilian P. de Courten. “The global epidemiology of non-insulin-dependent diabetes mellitus and the metabolic syndrome.” Journal of Diabetes and its Complications 11.2 (1997): 60-68.
6. American Diabetes Association. “Type 2 diabetes in children and adolescents.”Pediatrics 105.3 (2000): 671-680.
7. Saltiel, Alan R., and C. Ronald Kahn. “Insulin signalling and the regulation of glucose and lipid metabolism.” Nature 414.6865 (2001): 799-806.
8. Browning, Jeffrey D., and Jay D. Horton. “Molecular mediators of hepatic steatosis and liver injury.” Journal of Clinical Investigation 114.2 (2004): 147-152.
9. Aronoff, Stephen L., et al. “Glucose metabolism and regulation: beyond insulin and glucagon.” Diabetes Spectrum 17.3 (2004): 183-190.
10. Ross, E. J., and D. C. Linch. “Cushing’s syndrome—killing disease: discriminatory value of signs and symptoms aiding early diagnosis.” The Lancet320.8299 (1982): 646-649.
11. Levin, Marvin E., Vincenza C. Boisseau, and Louis V. Avioli. “Effects of diabetes mellitus on bone mass in juvenile and adult-onset diabetes.” New England Journal of Medicine 294.5 (1976): 241-245.
12. Sherwin, Robert, and Philip Felig. “Pathophysiology of diabetes mellitus.” The Medical clinics of North America 62.4 (1978): 695-711.
13. Faber, O. K., and C. Binder. “C-peptide response to glucagon: a test for the residual β-cell function in diabetes mellitus.” Diabetes 26.7 (1977): 605-610.
14. Ioannidis, Ioannis. “Pathophysiology of Type 1 diabetes.” Diabetes in Clinical Practice: Questions and Answers from Case Studies 31 (2007): 23.
15. Scheen, A. J. “Pathophysiology of type 2 diabetes.” Acta Clinica Belgica 58.6 (2003): 335-341.
16. Vambergue, A., et al. “[Pathophysiology of gestational diabetes].” Journal de gynecologie, obstetrique et biologie de la reproduction 31.6 Suppl (2002): 4S3-4S10.
17. Inzucchi, Silvio E. “Diagnosis of diabetes.” New England Journal of Medicine367.6 (2012): 542-550.
18. Turner, R. C., et al. “Intensive blood-glucose control with sulphonylureas or insulin compared with conventional treatment and risk of complications in patients with type 2 diabetes (UKPDS 33).” lancet 352.9131 (1998): 837-853.
19. Brownlee, M., and A. Cerami. “The biochemistry of the complications of diabetes mellitus.” Annual review of biochemistry 50.1 (1981): 385-432.
20. Pari, L., and G. Saravanan. “Antidiabetic effect of Cogent db, a herbal drug in alloxan-induced diabetes mellitus.” Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology 131.1 (2002): 19-25.
21. Day, Caroline. “Traditional plant treatments for diabetes mellitus: pharmaceutical foods.” British Journal of Nutrition 80.01 (1998): 5-6.
22. Smith, George Davey, et al. “Genetic epidemiology and public health: hope, hype, and future prospects.” The Lancet 366.9495 (2005): 1484-1498.
23. Ganesh G. Dhakad, Deepak S. Mohale, A.V. Chandewar. Evaluation of Anti-Diabetic Activity of Galinosa parviflora in Diabetic Rats. Res. J. Pharmacology and Pharmacodynamics.2024;16(3):143-152.
24. Beyene Derej, Aschalew Nardos, Jemal Abdela, Lidet Terefe, Melese Arega, Terfo Mikre Yilma, Tilahun Tesfaye. Antidiabetic Activities of 80% Methanol Extract and Solvent Fractions of Verbascum Sinaiticum Benth (Scrophulariaceae) Leaves in Mice. Journal of Experimental Pharmacology 2023:15 423–436.
25. Kolajo Adedamola Akinyede, Habeebat Adekilekun Oyewusi, Gail Denise Hughes, Okobi Eko Ekpo, and Oluwafemi Omoniyi Oguntibeju. In Vitro Evaluation of the Anti-Diabetic Potential of Aqueous Acetone Helichrysum petiolare Extract (AAHPE) with Molecular Docking Relevance in Diabetes Mellitus. Molecules 2022, 27, 155.
26. Nithiyaa Perumal, Meenakshii Nallappan, Shamarina Shohaimi, Nur Kartinee Kassim, Thiam Tsui Tee, Yew Hoong Cheah. Synergistic antidiabetic activity of Taraxacum officinale (L.) Weber ex F.H. Wigg and Momordica charantia L. polyherbal combination. Biomedicine & Pharmacotherapy 145 (2022) 112401.
27. Yuting Zhu , Hongwei Gao, Shanhao Han, Jianhui Li, Qiqin Wen, Bo Dong. Antidiabetic activity and metabolite profiles of ascidian Halocynthia roretzi. Journal of Functional Foods 93 (2022) 105095.
28. Aligwekwe A. Ugochukwu and Idaguko C. Anna, Phytochemical Evaluation and Anti-Diabetic Effects of Ethanolic Leaf Extract of Petersianthus macrocarpus on Streptozotocin-Induced Diabetic Rats. JAMMR, 33(3): 39-47, 2021.
29. Sharada Seekondaa and Roja Ranib. Evaluation of Antidiabetic and Histopathological Activities of Embelia robusta Seeds Extract against Alloxan-Induced Diabetic Wistar Rats. Dec 23, 2020.
30. M. Subaraman, R. Gayathri, V. Vishnu Priya. In vitro antidiabetic activity of crude acetone leaf extract of Annona muricata. Drug Invention Today | Vol 14 • Issue 6 • 2020.