Volume : 13, Issue : 01, January – 2026
Title:
STABILITY INDICATING METHOD DEVELOPMENT & VALIDATION OF LORCASERIN HYDROCHLORIDE BY RP-HPLC METHOD
Authors :
Pragati Ranjan Satpathy*, K Saravanan and Nilima Shukla
Abstract :
The current investigation aimed to develop and progressively validate a novel, simple, responsive, and stable RP-HPLC method for the Quantitative Determination of Lorcaserin Hydrochloride in active pharmaceutical ingredients and marketed pharmaceutical dosage forms. A simple, selective, validated and well-defined stability that shows isocratic RP-HPLC methodology for the quantitative determination of Lorcaserin Hydrochloride. The chromatographic strategy utilised a Symmetry ODS (C18) RP Column, 250 mm x 4.6 mm, 5µm, with isocratic elution using a mobile phase consisting of Phosphate Buffer (0.02 M) and Acetonitrile (pH 2.80) in a 60:40 v/v ratio. A flow rate of 1.0 ml/min and a detector wavelength of 225nm, utilising the UV detector, were given in the instrumental settings. Validation of the proposed method was carried out according to the International Conference on Harmonisation (ICH) guidelines. LOD and LOQ for the active ingredients were established with respect to test concentration. The calibration charts plotted were linear with a regression coefficient of R2>0.999, which means the linearity was within the limit. Recovery, specificity, linearity, accuracy, robustness, and ruggedness were determined as part of method validation, and the results were found to be within the acceptable range. The proposed method is to be fast, simple, feasible and affordable in assay condition. During stability tests, it can be used for routine analysis of the selected drugs.
Key Words:Lorcaserin Hydrochloride, RP-HPLC, Method Development, Validation, Accuracy
Cite This Article:
Please cite this article in press Pragati Ranjan Satpathy et al., Stability Indicating Method Development & Validation Of Lorcaserin Hydrochloride By Rp-Hplc Method, Indo Am. J. P. Sci, 2026; 13(01).
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