Volume : 13, Issue : 01, January – 2026

Title:

OCT4 AND SOX2 AS RADIOTHERAPY PREDICTIVE BIOMARKERS EFFECTIVENESS IN NORTH INDIAN PATIENTS WITH CERVICAL CANCER THE POPULATION

Authors :

Mr. Shashiranjan Yadav, Miss. Divyanshi Kushwah, Mr. Sinnet Mallik, Mr. Krishna Kumar Shah

Abstract :

Introduction: Cervical cancer is a leading cause of mortality among women, largely influenced by clinical and histopathological tumor characteristics at diagnosis. While tumor size is an effective predictor of chemoradiotherapy (CRT) response, variability in treatment outcomes highlights the need for reliable biomarkers. OCT4 and SOX2, key stem cell markers, have been linked to cancer progression, poor prognosis, and chemoresistance. This study aims to evaluate the expression of OCT4 and SOX2 in cervical cancer tissues and assess their potential as predictive biomarkers for radiotherapy efficacy.
Materials and Methods: The study included 56 cervical cancer patients, with detailed data on cancer stage, tumor characteristics, and treatment modalities collected from medical records. Tissue microarrays were constructed from paraffin-embedded samples, and immunohistochemical staining was performed to assess OCT4 and SOX2 expression, with staining intensity analyzed using specialized software. Histoscores were compared across subgroups, and ROC analysis determined cut-off values for biomarker expression. Kaplan-Meier survival analysis, Cox regression, and chi-square tests were used to evaluate associations between biomarker expression, survival, and disease characteristics, with statistical significance set at p < 0.05.
Results: The study found that 66.1% of cervical cancer patients achieved a complete response to radiotherapy, while 33.9% had a partial response. Higher SOX2 and OCT4 expression levels were significantly associated with partial response and some toxicities like diarrhea. SOX2 showed stronger predictive value for radiotherapy response compared to OCT4, with significant differences in expression between complete and partial response groups. ROC analysis highlighted SOX2 as a more reliable biomarker for predicting treatment outcomes. Conclusion: Higher expression of SOX2 and OCT4 was significantly associated with partial response to radiotherapy in cervical cancer patients, with SOX2 showing a stronger predictive value. These biomarkers may serve as useful tools for predicting radiotherapy efficacy and guiding personalized treatment strategies.
Keywords: Cervical cancer, OCT4, SOX2, Predictive biomarkers, Radiotherapy efficacy, Immunohistochemistry, Tissue microarray, Biomarker expression
.

Cite This Article:

Please cite this article in press Shashiranjan Yadav et al OCT4 AND SOX2 AS Radiotherapy Predictive Biomarkers Effectiveness In North Indian Patients With Cervical Cancer The Population, Indo Am. J. P. Sci, 2026; 13(01).

REFERENCES:

[1] Yim EK, Park JS. Department of Obstetrics and Gynaecology. Seoul, south Korea, Source: PubMed, published in Sage Journals. 2017 Nov. [LINK]
[2] Konstantina Papadatou, Nikolaos Thomakos, Alexandros Rodolakis. Section of Gynaecologic Oncology, published: January 2022.
[3] Yim E, Park J. Biomarkers in Cervical Cancer. Biomarker Insights. 2017 Nov 07;1:215-25. [PMID]
[4] Bhatla N, Lal N, Bao YP, Ng T, Qiao YL. A metaanalysis of human papillomavirus typedistribution in women from South Asia: implications for vaccination. Vaccine. 2008 Jun 2;26(23):2811-7. [DOI:10.1016/j.vaccine.2008.03.047]
[5] Liu SC, Huang EY, Hu CF, Ou YC, ChangChien CC, Wang CJ, Tsai CC, Fu HC, Wu CH, Lin H. Pretreatment factors associated with recurrence for patients with cervical cancer international federation of gynecology and obstetrics stage IB1 disease. Gynecologic and obstetric investigation. 2016 Nov 19;81(4):339-45. [DOI:10.1159/000441784]
[6] Rodriguez NM. Participatory innovation for human papillomavirus screening to accelerate the elimination of cervical cancer. The Lancet Global Health. 2021 May 1;9(5):e582-3. [DOI:10.1016/s2214-109x(20)30522-2
[7] Zhou J, Lei N, Tian W, Guo R, Chen M, Qiu L, Wu F, Li Y, Chang L. Recent progress of the tumor microenvironmental metabolism in cervical cancer radioresistance. Frontiers in Oncology. 2022 Oct 12;12:999643. [DOI:10.3389/fonc.2022.999643]
[8] Yang J, Cai H, Xiao ZX, Wang H, Yang P. Effect of radiotherapy on the survival of cervical cancer patients: An analysis based on SEER database. Medicine. 2019 Jul 1;98(30):e16421. [DOI:10.1097/md.0000000000016421]
[9] Chew JL, Loh YH, Zhang W, Chen X, Tam WL, Yeap LS, Li P, Ang YS, Lim B, Robson P, Ng HH. Reciprocal transcriptional regulation of Pou5f1 and Sox2 via the Oct4/Sox2 complex in embryonic stem cells. Molecular and cellular biology. 2005 Jul 1;25(14):6031-46.[DOI:10.1128/mcb.25.14.6031- 6046.2005]
[10] Nishimoto M, Fukushima A, Okuda A, Muramatsu M. The gene for the embryonic stem cell coactivator UTF1 carries a regulatory element which selectively interacts with a complex composed of Oct-3/4 and Sox-2. Molecular and cellular biology. 1999 Aug 1;19(8):5453-65. [DOI:10.1128/mcb.19.8.5453]
[11] Okumura-Nakanishi S, Saito M, Niwa H, Ishikawa F. Oct-3/4 and Sox2 regulate Oct-3/4 gene in embryonic stem cells. Journal of Biological Chemistry. 2005 Feb 18;280(7):5307-17. [DOI:10.1074/jbc.m410015200 ]
[12] Yuan H, Corbi N, Basilico C, Dailey L. Developmental-specific activity of the FGF-4 enhancer requires the synergistic action of Sox2 and Oct-3. Genes Dev. 1995;9:2635–45. [DOI: 10.1101/gad.9.21.2635]
[13] Schöler HR, Ruppert S, Suzuki N, Chowdhury K, Gruss P. New type of POU domain in germ linespecific protein Oct-4. Nature. 1990 Mar 29;344(6265):435-9. [DOI:10.1038/344435a0]
[14] Ponti D, Costa A, Zaffaroni N, Pratesi G, Petrangolini G, Coradini D, Pilotti S, Pierotti MA, Daidone MG. Isolation and in vitro propagation of tumorigenic breast cancer cells with stem/progenitor cell properties. Cancer research. 2005 Jul 1;65(13):5506-11. [DOI:10.1158/0008-5472.can-05-0626]
[15] Chiou SH, Wang ML, Chou YT, Chen CJ, Hong CF, Hsieh WJ, Chang HT, Chen YS, Lin TW, Hsu HS, Wu CW. Coexpression of Oct4 and Nanog enhances malignancy in lung adenocarcinoma by inducing cancer stem cell–like properties and epithelial–mesenchymal transdifferentiation. Cancer research. 2010 Dec 15;70(24):10433-44.. [DOI:10.1158/0008-5472.can-10-2638]
[16] Organista-Nava J, Gómez-Gómez Y, Gariglio P. Embryonic stem cell-specific signature in cervical cancer. Tumor Biology. 2014 Mar;35:1727-38. [DOI:10.1007/s13277-013-1321-y]
[17] Li X, Wang J, Xu Z, Ahmad A, Li E, Wang Y, Qin S, Wang Q. Expression of Sox2 and Oct4 and their clinical significance in human non-small-cell lung cancer. International journal of molecular sciences. 2012 Jun 21;13(6):7663-75. [DOI:10.3390/ijms13067663]
[18] Paul SB, Tiwary BK, Choudhury AP. Studies on the epidemiology of cervical cancer in Southern Assam. Assam University Journal of Science and Technology. 2011 Apr 6;7(1):36-42. [LINK]
[19] Shanthala S, Agadi BS, Kulkarni BB, Patil BR, Hallikeri UR, Chowkimath SM, Gai PB. Snap shot of epidemiological pattern of cervical cancer patients reporting to a tertiary cancer care center in North Karnataka, India. Int J Adva Biol Res. 2014;4(2):178-83.[LINK]
[20] Spartacus RK, Paliwal R, Gaur P, Mutha S, Bhatt Pr MA. A profile of cervical cancer cases in a government medical college hospital. IOSRJDMS. 2015;14(10):37-42. [LINK]
[21] Yumkhaibam SD, Ramalingam M, Singh IY, Singh TT, Singh LJ, Daru HK, Hage S. Clinicoepidemiological study of cervical carcinoma in Northeast India. Indian J Appl Res. 2016;6(8):64-6. [LINK]
[22] Wadhwani R, Bamnia R, Meena M. Clinicopathological analysis of cancer cervix in tertiary care centre. Journal of Evolution of Medical and Dental Sciences. 2013 Sep 30;2(39):7381-6. [LINK]
[23] Kaverappa VB, Prakash B, Kulkarni P, Renuka M. Sociodemographic profile of patients with cervical cancer in a tertiary-care cancer hospital in Mysuru, Karnataka. International Journal of Medical Science and Public Health. 2015 Sep 1;4(9):1187-91. [LINK]
[24] Singh R, Shetty N, Naveed M, Pawar S, Iska S, Alugubelli N. Retrospective analysis of patients with carcinoma cervix in a rural/semi-urban setting in Western India.