Volume : 13, Issue : 07, July – 2026

Title:

DEVELOPMENT OF HERBAL TRANSDERMAL PATCH USING OCIMUM TENUIFLORUM (TULSI) LEAF EXTRACT

Authors :

Aryan Chaudhary, Reetika Gupta*, Ashutosh Badola, Abhishek Bhardwaj, Dr Amandeep Singh

Abstract :

This study focuses on the formulation and evaluation of a herbal transdermal patch using Ocimum tenuiflorum (Tulsi) leaf extract, a plant well known for its medicinal, pharmacological, and therapeutic properties. Tulsi has been widely used in traditional systems of medicine, such as Ayurveda, for its antimicrobial, anti-inflammatory, antioxidant, and adaptogenic activities. The objective of this research was to develop a transdermal drug delivery system (TDDS) incorporating Tulsi extract to provide controlled and sustained release of its active constituents through the skin. The ethanolic extract of Tulsi leaves was prepared by the Soxhlet extraction method and subjected to phytochemical screening, which confirmed the presence of bioactive compounds such as glycosides, tannins, steroids, and polyphenols. The transdermal patches were formulated with hydroxypropyl methylcellulose (HPMC) and polyvinylpyrrolidone (PVP) as polymers, polyethene glycol (PEG) as a plasticiser, and prepared by solvent evaporation. The prepared patches were evaluated for physicochemical parameters including appearance, thickness, moisture content, folding endurance, and weight uniformity. The patches were found to be smooth, flexible, and uniform, with an average thickness of 0.523 mm, moisture content of 3.54%, folding endurance of 85, and average weight of 0.412 g. These findings indicate that the formulated patches possess satisfactory mechanical and physical properties and support the feasibility of Tulsi extract as an active ingredient in transdermal drug delivery.
Keywords: Ocimum tenuiflorum; Transdermal drug delivery system; Herbal transdermal patch; Phytochemical screening; Controlled drug release.

Cite This Article:

Please cite this article in press Reetika Gupta et al., Development Of Herbal Transdermal Patch Using Ocimum Tenuiflorum (Tulsi) Leaf Extract.,, Indo Am. J. P. Sci, 2026; 13(07).

REFERENCES:

1. Pandey G, Madhuri S. Pharmacological activities of Ocimum sanctum (Tulsi): A review. Int J Pharm Sci Rev Res. 2010;5(1):61.
2. Prajapati ND, Purohit SS, Sharma AK, Kumar T. A Handbook of Medicinal Plants. 1st ed. India: Agrobios; 2003. p. 367.
3. Pattanayak P, et al. Ocimum sanctum Linn: A reservoir plant for therapeutic applications – An overview. Pharmacogn Rev. 2010;4(7):95-107.
4. Bano N, Ahmed A. Pharmacological evaluation of Ocimum sanctum. J Bioequiv Availab. 2017;9(3):387-392.
5. Siva M, Shanmugam KR. Ocimum sanctum: A review on the pharmacological properties. Int J Basic Clin Pharmacol. 2016.
6. Kaur S, Sabharwal S. An overview of Tulsi (Holy Basil). Eur J Mol Clin Med. 2020;7(7).
7. Madhuri S. Studies on oestrogen induced uterine and ovarian carcinogenesis and effect of Prolmmu in rats [PhD thesis]. Jabalpur: Rani Durgavati Vishwa Vidyalaya; 2008.
8. Hardainiyan S, Nandy BC, Jasuja ND, Vyas P, Raghav KP. A review on the recent innovations in transdermal drug delivery for herbal therapy. J Biomed Pharm Res. 2014;3.
9. Savula J, Krishan Murali KS. Formulation and evaluation of herbal transdermal patches. World J Pharm Res. 2017;6(13):365-374.
10. Pandey G, Madhuri S. Autochthonous herbal products in the treatment of cancer. Phytomedica. 2006;7:99-104.
11. Ghulaxe C, Verma R. A review on transdermal drug delivery system. Pharma Innov J. 2015;4(1):37-43.
12. Xia ZK, Perveen N. Phytochemical analysis, antibacterial and antioxidant activity determination of Ocimum sanctum. Pharm Pharmacol Int J. 2018;6(6).
13. Dhiman S, Thakur GS. Transdermal patches: A recent approach to new drug delivery system. Int J Pharm Pharm Sci. 2011;3(Suppl 5).
14. Al Akeel R, Mateen A, Janardhan K, Gupta VC. Analysis of the anti-bacterial and antioxidative activity of Azadirachta indica bark using various solvent extracts. Saudi J Biol Sci. 2017;24:11-14.
15. Patel KD, Gidwani B. Formulation and evaluation of transdermal patch using antioxidant phytoconstituent. J Biol Sci. 2016;2(4).
16. Saleem NM, Idris M. Formulation design and development of a Unani transdermal patch for antiemetic therapy and its pharmaceutical evaluation. Scientifica. 2016.
17. Mishra P, Mishra S. Study of antibacterial activity of Ocimum sanctum extract against gram-positive and gram-negative bacteria. Am J Food Technol. 2011;6:336-341.
18. Sheth NN, Mistry RB. Formulation and evaluation of transdermal patches and to study permeation enhancement effect of eugenol. J Appl Pharm Sci. 2011;01(03):96-101.
19. Bathe R, Kapoor R. Transdermal drug delivery system: formulation, development and evaluation – An overview. Int J Biomed Adv Res. 2015;6(01):1-10.
20. Mukherjee PK. Quality control of herbal drugs: An approach to evaluation of botanicals. Business Horizons; 2007. p. 57-68, 217-220.
21. Vishwakarma AK, Maurya O. Formulation and evaluation of transdermal patch containing turmeric oil. Int J Pharm Pharm Sci. 2012;4(Suppl 5).
22. Mukherjee R, Das PK, Ram GC. Immunotherapeutic potential of Ocimum sanctum Linn. in bovine subclinical mastitis. Rev Vet Sci. 2005;79(1):37-43.
23. Ansari K, Singhai AK, Saraogi GK. Recent advancement in transdermal drug delivery system. Indian J Pharm Sci. 2011;3(Suppl 5):52-59.
24. Haripatil U, Gaikwad DK, et al. Int J Pharm Sci Res. 2011;2(3).
25. Joshi H, Parle M. Evaluation of nootropic potential of Ocimum sanctum Linn. in mice. Indian J Exp Biol. 2006;44:133-136.
26. Kumar R, Saha P, Lokare P. A systemic review of Ocimum sanctum (Tulsi): Morphological characteristics, phytoconstituents and therapeutic applications. Int J Res Appl Sci Biotechnol. 2022;9(2).
27. Anbarasu K, Vijayalakshmi G. Improved shelf life of protein-rich tofu using Ocimum sanctum (Tulsi) extracts to benefit Indian rural population. J Food Sci. 2007;72.
28. Shetty SS, Udupa S, Udupa A. Evaluation of antioxidant and wound healing effects of alcoholic and aqueous extract of Ocimum sanctum Linn. in rats. Evid Based Complement Alternat Med. 2008;5:95-110.
29. Srivastava M, Ghoshal S, Srivastava S, Srivastava AK, Maurya P. Herbal based transdermal patch drug delivery systems: Formulation strategies, challenges and future perspectives. Int J Pharm Biol Sci Arch.