Volume : 13, Issue : 06, June – 2026
Title:
METHOD DEVELOPMENT AND VALIDATION OF A ZERO-ORDER UV SPECTROPHOTOMETRIC METHOD FOR DETERMINATION OF APIXABAN
Authors :
Dr. Naveen Kumar G S, Jashwanth Gowda H U*
Abstract :
A simple, accurate, and precise zero order derivative UV spectroscopic method has been devised and validated for the estimation of Apixaban in bulk and tablet dosage form. According to Beer’s law, the concentration of Apixaban in methanol ranges from 4 to 24 µg/ml, and its absorbance reaches its maximum at 280 nm. The area under the curve in absorption spectra is measured between 275 and 285 nm. The regression coefficient (r2) was found to be 0.999 following a linearity investigation, demonstrating acceptable linearity and precision over this concentration range. The percentage recovery was found to be 99.2%, 101%, and 99.6%, while the limits of detection (LOD) and quantitation (LOQ) were found to be 1.09 and 3.3 micrograms per milliliter, respectively. Furthermore, excellent precision was indicated by the methodologies’ relative standard deviation (% RSD) values, which were less than 2%. All validation metrics, including linearity, accuracy, precision, ruggedness, LOD, and LOQ, were assessed in accordance with ICH requirements. The developed and proven method can be used to regularly estimate the dosage of Apixaban in both bulk and tablet form.
KEYWORDS: Apixaban ;Quantification;Validation; RP-HPLC; HPTLC, Anticoagulant.
Cite This Article:
Please cite this article in press Jashwanth Gowda H U et al., Method Development And Validation Of A Zero-Order Uv Spectrophotometric Method For Determination Of Apixaban.., Indo Am. J. P. Sci, 2026; 13(06).
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