Volume : 13, Issue : 06, June – 2026
Title:
QUALITY CONTROL AND ANTIOXIDENT PROFILING OF SEA BUCKTHORN (HIPPOPHAE RHAMNOIDES) CHURNA USING HPLC MARKER ANALYSIS AND DPPH ASSAY
Authors :
Manchare M.P, Rutuja Navnath Malusare ,Bhakti Gurav, Bhand R.B
Abstract :
The present study focuses on the quality control and antioxidant profiling of Sea Buckthorn Churna prepared from Hippophae rhamnoides using High Performance Liquid Chromatography (HPLC) marker analysis and DPPH free radical scavenging assay. Sea Buckthorn is a medicinal plant widely recognized for its rich content of bioactive compounds such as flavonoids, phenolic acids, vitamins, carotenoids, and antioxidants, which contribute to its therapeutic potential in various oxidative stress-related disorders. The increasing use of herbal formulations necessitates proper standardization and quality evaluation to ensure safety, efficacy, and consistency.
In the present investigation, Sea Buckthorn fruits were processed into churna form and subjected to physicochemical evaluation including organoleptic characteristics, ash values, moisture content, extractive values, and powder flow properties according to standard pharmacopoeial guidelines. HPLC marker analysis was performed for the identification and quantification of selected phytochemical markers to establish the chemical fingerprint profile of the formulation. The chromatographic study confirmed the presence of important phenolic and flavonoid constituents responsible for antioxidant activity.
The antioxidant potential of the prepared churna was evaluated using the DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging assay. The formulation exhibited significant free radical scavenging activity in a concentration-dependent manner, indicating strong antioxidant potential due to the presence of natural phytoconstituents. The results suggest that Sea Buckthorn churna possesses appreciable antioxidant properties and can serve as a valuable herbal formulation for combating oxidative stress.
The study concludes that HPLC marker analysis combined with DPPH assay provides an effective approach for the standardization, quality control, and antioxidant evaluation of Sea Buckthorn churna. The developed analytical profile may be useful for ensuring batch-to-batch consistency and promoting the scientific validation of herbal formulations.
Cite This Article:
Please cite this article in press Manchare M.P et al., Quality Control And Antioxident Profiling Of Sea Buckthorn (Hippophae Rhamnoides) Churna Using Hplc Marker Analysis And Dpph Assay.., Indo Am. J. P. Sci, 2026; 13(06).
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