Volume : 13, Issue : 06, June – 2026

Title:

IN-VITRO STUDY OF ETHANOLIC EXTRACT OF OCIMUM SANCTUM LEAVES FOR ANTI-CANCER, ANTI-DIABETIC, AND ANTI-INFLAMMATORY ACTIVITY

Authors :

Kamble Sanket Dattu, Dasrao Ashok Patil , Dr. Kavaljit Satish Birajdar

Abstract :

Ocimum sanctum, commonly known as Tulsi, is an important medicinal plant extensively used in traditional systems of medicine because of its diverse pharmacological properties. The present study was carried out to evaluate the in-vitro anticancer, anti-diabetic, and anti-inflammatory activities of the ethanolic extract of Ocimum sanctum leaves. The leaves were collected, shade dried, powdered, and extracted using ethanol through the solvent extraction method. The prepared extract was subjected to various in-vitro biological assays for the evaluation of its therapeutic potential.
The anticancer activity of the extract was assessed using suitable cell line studies, where it exhibited significant cytotoxic activity against cancer cells in a dose-dependent manner. Anti-diabetic activity was evaluated by α-amylase and α-glucosidase inhibition assays, which showed effective inhibition of carbohydrate metabolizing enzymes, indicating its potential in blood glucose regulation. The anti-inflammatory activity was studied by protein denaturation and membrane stabilization methods, and the extract demonstrated considerable inhibition of inflammatory responses.
The findings of the study indicate that the ethanolic extract of Ocimum sanctum leaves possesses significant pharmacological activities, which may be attributed to the presence of phytoconstituents such as flavonoids, tannins, phenolic compounds, and alkaloids. Thus, Ocimum sanctum can be considered a promising natural source for the development of herbal medicines. Further in-vivo and clinical investigations are necessary to establish its therapeutic safety and efficacy.
Keywords: Ocimum sanctum, Tulsi, Ethanolic extract, Anticancer activity, Anti-diabetic activity, Anti-inflammatory activity, In-vitro study, Phytochemicals, Herbal medicine.

Cite This Article: