Volume : 13, Issue : 03, March – 2026
Title:
DNA BARCODING AND MOLECULAR PHARMACOGNOSY: EMERGING TOOLS FOR AUTHENTICATION OF MEDICINAL PLANTS
Authors :
Shashikant Shivanand Langote, Divya Subhash Pawar, Sanika Sanjaykumar Gurav, Kavita Vishnu Shinde*, Priyanka Dhananjay Bankar, -Pragati gund, Pruthviraj Haridas Gund
Abstract :
Medicinal plants have long been recognized as an important source of therapeutic agents and continue to play a significant role in traditional and modern healthcare systems. A large proportion of the global population relies on herbal medicines for the prevention and treatment of various diseases. However, the growing demand for herbal products has raised serious concerns regarding the authenticity, quality, and safety of medicinal plant materials. Problems such as misidentification, adulteration, and substitution frequently occur during the collection, processing, and commercialization of herbal drugs, which may reduce therapeutic efficacy and pose potential health risks. Conventional pharmacognostic methods including morphological, microscopic, and phytochemical evaluations are commonly used for the identification of medicinal plants, but these approaches often show limitations, particularly when plant materials are processed or closely related species exhibit similar characteristics.
In recent years, molecular approaches have emerged as reliable tools for the authentication of medicinal plants. Among these, DNA Barcoding has gained considerable attention as a rapid and accurate method for species identification using standardized DNA sequences. DNA barcoding utilizes specific genetic markers such as rbcL, matK, ITS, and trnH-psbA to differentiate plant species based on their unique genetic signatures. These molecular techniques enable the identification of plant materials even in powdered or processed forms and facilitate the detection of adulterants in herbal products.
This review highlights the importance of molecular pharmacognosy and DNA barcoding in medicinal plant authentication, discusses commonly used genetic markers, and outlines recent technological advancements and applications in herbal drug quality control. Integration of molecular tools with conventional pharmacognostic methods can significantly improve the accuracy, safety, and standardization of herbal medicines.
Keywords: DNA barcoding; Molecular pharmacognosy; Medicinal plant authentication; Herbal drug quality control; Genetic markers; rbcL; matK; ITS; Adulteration detection; Herbal medicines.
Cite This Article:
Please cite this article in press Kavita Vishnu Shinde et al., Dna Barcoding And Molecular Pharmacognosy: Emerging Tools For Authentication Of Medicinal Plants., Indo Am. J. P. Sci, 2026; 13(03).
REFERENCES:
1. Chen S, Pang X, Song J, Shi L, Yao H, Han J, et al. DNA barcoding of medicinal plants and its applications in herbal medicine identification. Plant Methods. 2018;14:1–10.
2. Raclariu AC, Heide M, Ichim MC, de Boer HJ. DNA metabarcoding for quality control of herbal medicines. Sci Rep. 2018;8:1–10.
3. Mishra P, Kumar A, Nagireddy A, Mani DN, Shukla AK, Tiwari R, et al. DNA barcoding: An efficient tool to overcome authentication challenges in medicinal plants. Biotechnol Lett. 2018;40:815–826.
4. Parveen I, Gafner S, Techen N, Murch SJ, Khan IA. DNA barcoding for the identification of botanical ingredients in herbal medicines. Planta Med. 2019;85:6–15.
5. de Boer HJ, Ichim MC, Newmaster SG. DNA barcoding and pharmacovigilance of herbal medicines. Drug Saf. 2019;42:745–754.
6. Newmaster SG, Grguric M, Shanmughanandhan D, Ramalingam S, Ragupathy S. DNA barcoding detects contamination and substitution in herbal products. BMC Med. 2019;17:1–12.
7. Ganie SH, Upadhyay P, Das S, Prasad A. Authentication of medicinal plants by DNA markers. Plant Gene. 2019;19:100189.
8. Ichim MC. The DNA-based authentication of commercial herbal products reveals widespread substitution. Front Pharmacol. 2019;10:1227.
9. Sánchez M, González-Burgos E, Iglesias I, Gómez-Serranillos MP. DNA-based authentication and metabolomics analysis for medicinal plant quality control. Plants. 2020;9:1601.
10. Seethapathy GS, Ganesh D, Kumar JU, Newmaster SG, Ragupathy S. DNA barcoding identifies contamination in herbal products in the market. Sci Rep. 2020;10:1–9.
11. Gao Z, Liu Y, Wang X, Wei X, Han J. DNA barcoding technology in herbal medicine identification. Front Plant Sci. 2020;11:570.
12. Techen N, Parveen I, Pan Z, Khan IA. DNA barcoding of medicinal plant material for identification. Curr Opin Biotechnol. 2020;61:103–110.
13. Kumar S, Ved DK, Tiwari R. Molecular pharmacognosy: Applications in herbal drug authentication. J Ethnopharmacol. 2020;260:113018.
14. Mahgoub YA, Al-Anazi KM, Al-Zahrani HS. Integration of DNA barcoding and metabolomics for medicinal plant authentication. Food Control. 2022;135:108802.
15. Zhu S, Fushimi H, Cai S, Komatsu K. DNA barcoding as an effective technique for identifying herbal medicines. Chinese Med. 2022;17:1–12.
16. Noviana E, Setyawan AD, Sugiyarto. Advances in fingerprint analysis for herbal drug standardization. Front Pharmacol. 2022;13:853023.
17. Paksoy MY, Duran A, Aytaç Z. DNA barcoding and phylogenetic analysis of medicinal plant species. Not Bot Horti Agrobo. 2022;50:12900.
18. Chen S, Pang X, Yao H. DNA barcoding in herbal medicine: Retrospective and prospective perspectives. Chinese Med. 2023;18:59.
19. Safhi FA, Al-Mutairi KA, Al-Sogair FM. DNA barcoding identification of wild medicinal plants. Genes. 2023;14:469.
20. Nazar N, Khan M, Ahmad M. Integrating DNA barcoding for herbal product authentication. Phytochem Anal. 2024;35:215–227.
21. Letsiou S, Konstantinidis T. DNA barcoding as a plant identification method: Advances and future perspectives. Appl Sci. 2024;14:1415.
22. Yong WTL, Lee JY. DNA barcoding using matK and rbcL markers for plant species identification. Plant Diversity. 2024;46:45–53.
23. Kim DY, Lee JH, Park JH. Authentication of ginseng supplements using DNA barcoding and multiplex PCR. Food Control. 2025;160:110336.
24. Xin T, Li X, Yao H. Application of DNA barcoding for monitoring medicinal plant authenticity in supply chains. Ind Crops Prod. 2025;198:116655.
25. Aswin PP, Nair RK. Enhancing authentication: DNA barcoding for detecting adulterants in herbal medicines. Microchem J. 2025;220:116655.
26. Narayana SKK, Prasad A, Ragupathy S. Integrating macro-microscopy and DNA markers for medicinal plant authentication. Plants. 2025;14:1254.
27. Balaji R, Parani M. DNA barcoding of medicinal plants: Advances and applications in herbal drug authentication. Plant Cell Biotechnol Mol Biol. 2018;19:1–10.
28. Vassou SL, Kusuma G, Parani M. DNA barcoding for species identification in herbal medicine. Gene. 2018;642:1–12.
29. Raclariu AC, Heinrich M, Ichim MC, de Boer HJ. Benefits and limitations of DNA barcoding in herbal product authentication. Pharm Biol. 2018;56:123–128.
30. Ghorbani A, Saeidnia S, Abdullah NR. DNA barcoding for medicinal plant identification: A systematic review. Phytochem Rev. 2019;18:1–20.
31. Ichim MC, de Boer HJ. A review of authenticity testing of herbal products using DNA-based techniques. Drug Saf. 2019;42:745–760.
32. Mishra P, Kumar A, Shukla AK, Sundaresan V. DNA barcoding in herbal drug authentication: Current perspectives. J Appl Res Med Aromat Plants. 2019;12:1–10.
33. Ganie SH, Das S, Prasad A. Molecular markers in medicinal plant authentication. Plant Gene. 2019;19:100189.
34. Seethapathy GS, Ganesh D, Kumar JU, Newmaster SG, Ragupathy S. Assessing product adulteration in herbal medicines using DNA barcoding. Sci Rep. 2020;10:1–10.
35. Techen N, Parveen I, Khan IA. DNA barcoding in botanical drug identification and quality control. Curr Opin Biotechnol. 2020;61:103–110.
36. Gao Z, Liu Y, Wang X, Wei X, Han J. DNA barcoding technology in herbal medicine identification. Front Plant Sci. 2020;11:570.
37. Kumar S, Ved DK, Tiwari R. Molecular pharmacognosy approaches for herbal drug authentication. J Ethnopharmacol. 2020;260:113018.
38. Frigerio J, Raclariu AC, Heinrich M, de Boer HJ. DNA metabarcoding for herbal product surveillance. Front Pharmacol. 2020;11:1–12.
39. Xin T, Yao H, Gao H. Super-barcode and genome skimming approaches for plant authentication. Plant Biotechnol J. 2021;19:1–13.
40. Kress WJ, Erickson DL. DNA barcodes in plant biodiversity studies. Proc Natl Acad Sci USA. 2021;118:e2102218118.
41. Shanmughanandhan D, Ragupathy S, Newmaster SG. DNA barcoding in quality control of herbal medicines. BMC Genomics. 2021;22:1–11.
42. Urumarudappa SKJ, Gogna N, Newmaster SG. Development of DNA barcode library for medicinal plants. Sci Rep. 2022;12:1–12.
43. Zhu S, Fushimi H, Cai S, Komatsu K. DNA barcoding as an effective technique for identifying herbal medicines. Chinese Med. 2022;17:1–12.
44. Mahgoub YA, Al-Anazi KM, Al-Zahrani HS. Integration of metabolomics and DNA barcoding for medicinal plant authentication. Food Control. 2022;135:108802.
45. Noviana E, Setyawan AD, Sugiyarto. Advances in analytical fingerprinting for herbal drug standardization. Front Pharmacol. 2022;13:853023.
46. Paksoy MY, Duran A, Aytaç Z. DNA barcoding and phylogenetic analysis of medicinal plants. Not Bot Horti Agrobo. 2022;50:12900.
47. Chen S, Pang X, Yao H. DNA barcoding in herbal medicine: Retrospective and prospective perspectives. Chinese Med. 2023;18:59.
48. Safhi FA, Al-Mutairi KA, Al-Sogair FM. DNA barcoding identification of wild medicinal plants. Genes. 2023;14:469.
49. Letsiou S, Konstantinidis T. DNA barcoding as a plant identification method: Advances and applications. Appl Sci. 2024;14:1415.
50. Yong WTL, Lee JY. DNA barcoding using matK and rbcL markers for plant species identification. Plant Divers. 2024;46:45–53.
51. Nazar N, Khan M, Ahmad M. Integrating DNA barcoding for herbal product authentication. Phytochem Anal. 2024;35:215–227.
52. Kim DY, Lee JH, Park JH. Authentication of herbal supplements using DNA barcoding and PCR analysis. Food Control. 2025;160:110336.
53. Chen R, Liu Y, Zhao Z. Recent progress in DNA-based approaches for herbal authentication. J Pharm Biomed Anal. 2025;235:115495.
54. Zhou S, Zhang X, Wang Y. DNA metabarcoding unveils authenticity and adulteration in herbal formulations. Front Pharmacol. 2025;16:1584065.
55. Aswin PP, Nair RK. DNA barcoding approach for detecting adulterants in medicinal plants. Microchem J. 2025;220:116655.
56. Raj SLA. DNA barcoding as a tool for plant species authentication and biodiversity conservation. Plant Sci Today. 2025;12:45–52.




