Volume : 13, Issue : 10, October – 2026

Title:

FORMULATION AND EVALUATION OF DIFAMILAST LOADED TRANSFEROSOMES FOR ENHANCED TOPICAL SKIN THERAPY

Authors :

Sowjanya Daniel*, Arshiya, Gayintha Pranitha, Kesani Abhilash, Modala Sai Prasad, Nomula Mamatha

Abstract :

The present study was undertaken to formulate and evaluate Difamilast-loaded transferosomes for enhanced transdermal drug delivery. Difamilast, a phosphodiesterase-4 (PDE-4) inhibitor used in the management of atopic dermatitis, possesses limited skin permeation when administered conventionally. FTIR studies demonstrated the compatibility of the drug with selected excipients, indicating the absence of any significant chemical interaction. The prepared transferosomal formulations exhibited particle sizes ranging from 122 to 135 nm and zeta potential values between −19 mV and −29 mV, indicating nanosized vesicles with good physical stability. Entrapment efficiency was found to range from 79.63% to 84.20%, confirming efficient drug incorporation within the vesicular system. In vitro diffusion studies revealed sustained drug release from all formulations over a period of 8 hours. Among the developed formulations, F4 exhibited the highest cumulative drug release (98.84%) and maximum entrapment efficiency (84.20%), indicating superior formulation performance. SEM analysis confirmed the formation of spherical, smooth, and non-aggregated transferosomal vesicles. Stability studies conducted on the optimized formulation (F4) for three months under different storage conditions showed no significant changes in drug release characteristics, confirming the stability of the formulation. The results suggest that Difamilast-loaded transferosomes are a promising vesicular drug delivery system capable of enhancing drug entrapment, improving stability, and providing sustained transdermal drug release, thereby offering potential therapeutic advantages for topical treatment.
Keywords: Difamilast, Transferosomes, Transdermal Drug Delivery, Vesicular System, Entrapment Efficiency, Zeta Potential, In Vitro Drug Release, Stability Studies.
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Cite This Article:

Please cite this article in press Sowjanya Daniel et al., Formulation And Evaluation Of Difamilast Loaded Transferosomes For Enhanced Topical Skin Therapy.,. Indo Am. J. P. Sci, 2026; 13(10).

REFERENCES:

1. Kumar KP., Radhika PR., Sivakumar T., Ethosomes: A Priority in Transdermal Drug Delivery, International Journal of Advances in Pharmaceutical Sciences 2010;1:111-121.
2. Heeremans JLM., Gerristen HR., Meusen SP., Mijnheer FW., Gangaram RS., Panday G., Prevost R., Kluft C., Crommelin DJA., The preparation of tissue type plasminogen activator (t- PA) containing liposomes: entrapment efficacy and ultracentrifugation damage, Journal of Drug Targeting 1995;3:301.
3. Asbill CS., El-Kattan AF., Michniak B., Enhancement of transdermal drug delivery: chemical and physical approaches, Critical Reviews in Therapeutic Drug Carrier Systems 2000;17:621.
4. Touitou E., Dayan N., Levi-Schaffer F., Piliponsky A., Novel lipid vesicular system for enhanced deliveryJournal of Lipid Research1998;8:113.
5. Verma P., Pathak K., Therapeutic and cosmeceutical potential of ethosomes: An overview, Journal of Advanced Pharmaceutical Technology & Research 2010;1:274-82.
6. Jain S., Umamaheshwari RB., Bhadra D., Jain NK., Ethosomes: A novel vesicular carrier for enhanced transdermal delivery of an anti-HIV agent, Indian Journal of Pharmaceutical Sciences 2004;66:72-81.
7. Touitou E., Godin B., Dayan N., Weiss C., Piliponsky A., Levi-Schaffer F., Intracellular delivery mediated by an ethosomal carrier, Biomaterials 2001;22:3053-3059.
8. Manosroi A., Jantrawut P., Khositsuntiwong N., Manosroi W., Manosroi J., Novel Elastic Nano vesicles for Cosmeceutical and Pharmaceutical Applications, Chiang Mai Journal of Science 2009;36,2:168-178.
9. Rakesh R., Anoop KR., Ethosome for Transdermal and Topical Drug Delivery, International Journal of Pharmaceutical Sciences and Research 2012;4,3:17-24.
10. Gangwar S., Singh S., Garg G., Ethosomes: A Novel Tool for Drug Delivery Through the Skin, Journal of Pharmacy Research 2010;3,4:688-691.
11. Jain H., Patel J., Joshi K., Patel P., Upadhyay UM., Ethosomes: A Novel Drug Carrier, International Journal of Clinical Practice 2011;7:1:1-4.
12. Upadhyay N., Mandal S., Bhatia L., Shailesh S., Chauhan P., A Review on Ethosomes: An Emerging Approach for Drug Delivery through the Skin, Recent Research in Science and Technology 2011;3,7:19-24. Divya * et al./ Int. J. Pharm. Med. Res. 2016; 4(4):354-363 362 13.
13. Sivakranth M., AnjumaAra P., Krishnaveni C., Venkatesh E., Ethosomes: A Novel Vesicular Drug Delivery System, International Journal of Advances in Pharmaceutical Research 2012;2,1:16-27.
14. Kumar R., Aslam MD., Tripathi A., Prasad D., Chaudhary V., Jain V., Mishra SK., Singh R., Ethosomes: Novel Vesicular Carriers in Transdermal Drug Delivery, Journal of Global Pharma Technology 2010;2,6:1-7.
15. Rathore AR., Khambete H., Jain S., Preparation and Characterization of Repaglinide Loaded Ethosomal Gel for the Treatment of NIDDM, International Journal of Pharmaceutical and Biological Archives 2013;4,2:385- 390.
16. Shahwal V., Samnani A., Dubey B., Bhowmick M., Ethosomes: An Overview, International Journal of Biomedical and Advance Research 2011;2: 161-168.
17. Touitou E., Drug delivery Across Skin, Expert Opinion on Biological Therapy 2002;2:723-733.
18. Schreier H., Bovwstra J., Liposomes and Niosomes as Topical Drug Carriers: Dermal and Transdermal Drug Delivery, Journal of Control Release 1994;30:1-15.