Volume : 13, Issue : 10, October – 2026

Title:

VALIDATED RP-HPLC METHOD FOR SIMULTANEOUS ESTIMATION OF LOSARTAN AND AMLODIPINE IN BULK AND TABLET DOSAGE FORM

Authors :

Akula Srilekha, K. Usha

Abstract :

A simple, precise, and validated Reverse Phase-High Performance Liquid Chromatography (RP-HPLC) method was developed for the simultaneous estimation of Losartan and Amlodipine in bulk and tablet dosage forms. The chromatographic analysis was carried out using a Develosil C18 column (4.6 mm × 250 mm, 5 µm) maintained at ambient temperature. The mobile phase comprised Acetonitrile and Acetate buffer (pH 4.3) in the ratio of 35:65% v/v, delivered at a flow rate of 1 mL/min. Detection was performed at a wavelength of 238 nm, with an injection volume of 20 µL and a run time of 6 minutes.
The method produced well-resolved peaks for both Losartan and Amlodipine with consistent retention times and no interference from excipients. It was validated in accordance with ICH Q2(R1) guidelines, demonstrating excellent linearity, precision, accuracy, specificity, and robustness. The results confirm that this RP-HPLC method is suitable for the routine quality control of Losartan and Amlodipine in combined pharmaceutical dosage forms.
Keywords: RP-HPLC, Losartan, Amlodipine, Develosil C18 column, linearity, accuracy.

Cite This Article:

Please cite this article in press Akula Srilekha et al., Validated Rp-Hplc Method For Simultaneous Estimation Of Losartan And Amlodipine In Bulk And Tablet Dosage Form.,. Indo Am. J. P. Sci, 2026; 13(10).

REFERENCES:

1. Rao BV, Sowjanya GN, Ajitha A, Rao Uma MV. A review on stability indicating HPLC method development, World journal of pharmacy and pharmaceutical sciences.2015; 4(8):405-423.
2. Rajan HV. Development and validation of HPLC method – A Review. International Journal of current research in pharmacy. 2015;1(2):55-68.
3. 3. Kumar V, Bharadwaj R, Gupta G, Kumar S. An Overview on HPLC Method Development, Optimization and Validation process for drug analysis. The Pharmaceutical and Chemical Journal. 2015; 2(2):30-40.
4. Gupta V, Jain AD, Gill NS, Gupta K. Development and validation of HPLC method – a review. International Research Journal of Pharmaceutical and Applied Sciences. 2012; 2(4):17-25.
5. Sonia K, Nappinnai M. Development and validation of HPLC and UV-visible spectrophotometric method for the pharmaceutical dosage form and biological fluid –review. European Journal of Biomedical and Pharmaceutical sciences. 2016; 3(3): 382-391.
6. Sánchez MLF. Chromatographic techniques, European RTN Project, GLADNET, retrieved on 05-09-2013.
7. Snyder LR, Kirkland JJ, Glach JL. Practical HPLC Method Development, John Wiley and Sons, New York, 1997; 158-192.
8. HPLC – Chemiguide. May 2, 2007. www.chemguide.co.uk
9. Rao G, Goyal A. An Overview on Analytical Method Development and Validation by Using HPLC. The Pharmaceutical and Chemical Journal, 2016; 3(2): 280-289.
10. McpolinOona.an Introduction to HPLC for Pharmaceutical Analysis. Mourne Training Service. 11-12.