Volume : 13, Issue : 09, September – 2026
Title:
FORMULATION AND INVITRO EVALUTION OF ARTEMISIA ARBORESCENS EXTRACT LOADED SOLID LIPID NANOPARTICLES
Authors :
Venkatalakshmi. K*, N. Mounika, Adeeba Siddiqua, P. Sirija, P. Avinash Kumar
Abstract :
The present study aimed to formulate and evaluate Artemisia arborescens-loaded Solid Lipid Nanoparticles (SLNs) to enhance drug stability, sustain drug release, and improve therapeutic efficacy. Four formulations (F1–F4) were prepared using varying concentrations of formulation components and evaluated for their physicochemical characteristics, drug-excipient compatibility, particle size, zeta potential, entrapment efficiency, in vitro drug release, and stability. The particle size of the formulations ranged from 121 to 164 nm, confirming their nanometric nature, while zeta potential values ranged from −25 to −36 mV, indicating good physical stability. Among all formulations, F3 was identified as the optimized formulation, exhibiting a particle size of 135 nm, entrapment efficiency of 81.24%, and zeta potential of −32 mV. In vitro drug release studies demonstrated a sustained release pattern over 8 hours, with F3 showing the highest cumulative drug release of 95.74%. Stability studies performed under different storage conditions for three months revealed no significant changes in the physicochemical properties or drug release profile of the optimized formulation, with drug release remaining above 92% throughout the study period.The findings of this study suggest that Artemisia arborescens-loaded SLNs represent a promising nanocarrier system capable of improving drug stability and providing sustained drug release, thereby potentially enhancing therapeutic effectiveness. The optimized formulation (F3) may serve as a suitable candidate for further in vivo investigations and future pharmaceutical applications.
Keywords: Artemisia arborescens, Solid lipid Nano Particles, homogenizing technique, FTIR, In-vitro drug release.
Cite This Article:
Please cite this article in press Venkatalakshmi. K et al., Formulation And Invitro Evalution Of Artemisia Arborescens Extract Loaded Solid Lipid Nanoparticles.,. Indo Am. J. P. Sci, 2026; 13(09).
REFERENCES:
1. S. Mukherjee, S. Ray and R. S. Thakur, Ind. J. Pharm. Sci., 349-358 (2009).
2. M. R. Mozafari, 41-50 (2006).
3. Rainer H. Muller, Karsten Mader and Sven Gohla, Eur. J. Pharm. Biopharm., 50(1), 161- 177 (2000).
4. Wolfgang Mehnart and Karsten Mader, Adv. Drug. Deliv. Rev., 47, 165-196 (2001).
5. Houli Li, Xiaobin Zhao, Yukun Ma and Guangxi Zhai, Ling Bing Li and Hong Xiang, Lou. J. Cont. Release, 133, 238-244 (2009).
6. Melike Uner, Gulgun Yener, Int. J. Nanomedicine, 2(3), 289-300 (2007).
7. Annette Zur Mehlen, Cora Schwarz and Wolfgang Mehnart, Eur. J. Pharm. Biopharm., 45, 149-155 (1998).
8. Elena Ugazia, Roberta Cavalli and M. R. Gasco, Int. J. Pharm., 241, 341-344 (2002).
9. Indu Pal Kaur, Rohit Bhandari, Swati Bhandari and Kakkur. J. Cont. Rel., 127,97-109 (2008).
10. Ghada Abdelbary and Rania H. Fahmy, AAPS Pharm. Sci. Tech., 10(1) (2009).
11. Heiati H, Tawashi R, Phillips NC. Solid lipid nanoparticles as drug carriers – II. Plasma stability and bio-distribution of solid lipid noparticles containing the lipophilic prodrug 3’azido-3′-deoxythymidine palmitate in mice, Int. J. Pharm. 1997; 149:255-265.
12. Chen H, Chang X, Du D, Liu, W, Liu J, Weng T, Yang Y, Xu H, Yang X. Podophyllotoxin-loaded solid lipid nanoparticles for epidermal targeting J. Control. Rel. 2006; 110: 296-306.
13. Cavalli R, Caputo O, Marengo E, Pattarino F, Gasco MR. The effects of components of micro emulsions on both size and crystalline structure of solid lipid nanoparticles (SLN) containing a series of model molecules. Pharmazie, 1998; 53: 392-396.
14. Zur Mühlen A, Mehnert W. Drug release and release mechanism of prednisolone loaded solid lipid nanoparticles. Pharmazie 1998; 53:552.
15. Levy MY, Benita S. Drug release from submicronized o/w emulsion: a new in vitro kinetic evaluation model. Int. J. Pharm.1990; 66: 29-37.
16. Mueller RH, Solid lipid nanoparticles (SLN) for controlled drug delivery – a review of the state of the art. Eur. J. Pharm. Biopharm. 2000; 50:161-177.
17. Cavalli R, Gasco MR, Chetoni P, Burgalassi S, Saettone MF. Solid lipid nanoparticles (SLN) as ocular delivery system for tobramycin, Int. J. Pharm. 2002; 15:241-5.