Volume : 13, Issue : 09, September – 2026
Title:
IN VITRO ANTHELMINTIC ACTIVITY OF PSIDIUM GUAJAVA L. EXTRACTS AGAINST PHERETIMA POSTHUMA
Authors :
Amena banu, Poonam, Mohammed Younus, Bibi sara, Nazaneen and Hanamanth
Abstract :
Background: Helminthic infections remain an important parasitic health problem, while resistance to established anthelmintic agents has encouraged investigation of plant-derived alternatives. Psidium guajava L. (Myrtaceae) is a medicinal plant reported in the source study to contain flavonoids, tannins, saponins, triterpenoids and other bioactive constituents. Objective: To evaluate the in vitro anthelmintic activity of petroleum ether and aqueous extracts of P. guajava against Pheretima posthuma using paralysis time (PT) and death time (DT) as outcome measures. Methods: Plant material was shade-dried, powdered and extracted using petroleum ether and water. Physicochemical parameters, preliminary phytochemical screening and an acute oral toxicity assessment were reported. Adult P. posthuma were exposed to extract concentrations of 25, 50, 75 and 100 mg/mL and compared with albendazole (10 mg/mL) and control treatment. Results: Petroleum ether extract produced PT/DT values of 90.3/100.0, 70.3/90.3, 65.8/85.8 and 56.1/70.3 min at 25, 50, 75 and 100 mg/mL, respectively. The aqueous extract produced PT/DT values of 81.3/90.1, 49.3/60.2, 44.8/55.8 and 26.1/30.9 min at the same concentrations. Albendazole produced PT/DT values of 20.1/26.4 min. Conclusion: Both P. guajava extracts demonstrated concentration-associated reductions in paralysis and death times in the reported assay, with the aqueous extract showing the shorter times at the tested concentrations. The findings support further investigation of P. guajava constituents and mechanisms responsible for the observed activity. Keywords:Psidium guajava; anthelmintic activity; Pheretima posthuma; aqueous extract; petroleum ether extract; albendazole; phytochemical screening.
Cite This Article:
Please cite this article in press Amena Banu et al., In vitro anthelmintic activity of psidium guajava l. Extracts against pheretima posthuma..,. Indo Am. J. P. Sci, 2026; 13(09).
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