Volume : 13, Issue : 09, September – 2026

Title:

EVALUATION OF HYPOLIPIDEMIC POTENTIAL OF TRICHOLOMA MATSUTAKE ON WISTAR ALBINO RATS

Authors :

Chintana B N*, Suresha B S, T Balasubramanian, Deepika J K

Abstract :

Hyperlipidemia is an important risk factor for cardiovascular diseases and is characterized by abnormalities in serum lipid levels. Tricholoma matsutake is an edible medicinal mushroom containing various bioactive constituents with potential health benefits. The present study was undertaken to evaluate the hypolipidemic activity of the ethyl acetate extract of T. matsutake (EAETM) in high-fat diet (HFD)-induced hyperlipidemic Wistar albino rats. The ethyl acetate extract was prepared by Soxhlet extraction. Thirty male Wistar albino rats were divided into five groups (n=6), and hyperlipidemia was induced using a high-fat diet consisting of vanaspati ghee and coconut oil (3:1, v/v) for six weeks. EAETM was administered orally at doses of 200 and 400 mg/kg during the final three weeks, while atorvastatin (10 mg/kg) was used as the standard drug. Serum total cholesterol (TC), triglycerides (TG), HDL-C, LDL-C, VLDL-C and atherogenic index (AI) were evaluated. The HFD produced a significant alteration in the serum lipid profile, characterized by increased TC, TG, LDL-C, VLDL-C and AI and decreased HDL-C. Treatment with EAETM significantly improved these parameters compared with the HFD control group. At 200 and 400 mg/kg, EAETM reduced TC from 139.51 ± 6.12 to 113.32 ± 6.69 and 104.62 ± 4.89 mg/dL, respectively, while TG decreased from 141.67 ± 5.81 to 118.84 ± 5.96 and 110.39 ± 5.97 mg/dL. HDL-C increased from 23.31 ± 1.54 to 33.80 ± 2.13 and 36.52 ± 1.92 mg/dL, respectively. LDL-C and AI were also reduced following EAETM treatment, with the 400 mg/kg dose showing greater numerical improvement. These findings indicate that EAETM possesses hypolipidemic activity in HFD-induced hyperlipidemic rats and may have potential as a natural source for lipid management. Further studies are required to establish the underlying mechanisms and therapeutic potential.
Keywords: Tricholoma matsutake, hypolipidemic activity, high-fat diet, Wistar rats, serum lipids.

Cite This Article:

Please cite this article in press Chintana B N et al., Evaluation Of Hypolipidemic Potential Of Tricholoma Matsutake On Wistar Albino Rats.,. Indo Am. J. P. Sci, 2026; 13(09).

REFERENCES:

1. Yao YS, Li TD, Zeng ZH. Mechanisms underlying direct actions of hyperlipidemia on myocardium: an updated review. Lipids in Health and Disease. 2020 Dec;19:1-6.
2. Alloubani A, Nimer R, Samara R. Relationship between hyperlipidemia, cardiovascular disease and stroke: a systematic review. Current cardiology reviews. 2021 Nov 1;17(6):52-66.
3. Udomkasemsab A, Prangthip P. High fat diet for induced dyslipidemia and cardiac pathological alterations in Wistar rats compared to Sprague Dawley rats. Clínica e Investigación en Arteriosclerosis. 2019 Mar 1;31(2):56-62.
4. Li H, Wang S, Wang S, Yu H, Yu W, Ma X, He X. Atorvastatin inhibits high-fat diet-induced lipid metabolism disorders in rats by inhibiting Bacteroides reduction and improving metabolism. Drug design, development and therapy. 2022 Jan 1:3805-16.
5. Kumar K, Mehra R, Guiné RP, Lima MJ, Kumar N, Kaushik R, Ahmed N, Yadav AN, Kumar H. Edible mushrooms: A comprehensive review on bioactive compounds with health benefits and processing aspects. Foods. 2021 Dec 4;10(12):2996.
6. Mustafa F, Chopra H, Baig AA, Avula SK, Kumari S, Mohanta TK, Saravanan M, Mishra AK, Sharma N, Mohanta YK. Edible mushrooms as novel myco-therapeutics: effects on lipid level, obesity, and BMI. Journal of Fungi. 2022 Feb 21;8(2):211.
7. Aref M, Khoshhali M, Ghasemi P, Adeli S, Heidari-Beni M, Kelishadi R. Effect of Ganoderma lucidum on serum lipid profiles: A systematic review and meta-analysis on animal studies. Journal of Res earch in Medical Sciences: The Official Journal of Isfahan University of Medical Sciences. 2023 Sep 29;28:70.
8. Yamanaka T, Yamada A, Furukawa H. Advances in the cultivation of the highly-prized ectomycorrhizal mushroom Tricholoma matsutake. Mycoscience. 2020;61(2):49-57.
9. Li M, Zhang Y, Lu Q, Gao Y, Ye T, Wang C, Xing D. Structure, bioactivities and applications of the polysaccharides from Tricholoma Matsutake: a review. Food Science and Technology. 2022 Jun 17;42:e44922.
10. Anusiya G, Gowthama Prabu U, Yamini NV, Sivarajasekar N, Rambabu K, Bharath G, Banat F. A review of the therapeutic and biological effects of edible and wild mushrooms. Bioengineered. 2021 Dec 20;12(2):11239-68.
11. Pu Y, Yin C, Zhang L, Fang X, Zhong X, Tian Y, Tao A, Yang Y, Xia C. Extraction, purification, structural characterization, pharmacological activities, and applications of Tricholoma polysaccharides: A review. Journal of Ethnopharmacology. 2025 Jun 26; 350:120053.
12. Geng X, Tian G, Zhang W, Zhao Y, Zhao L, Wang H, Ng TB. A Tricholoma matsutake peptide with angiotensin converting enzyme inhibitory and antioxidative activities and antihypertensive effects in spontaneously hypertensive rats. Scientific reports. 2016 Apr 7;6(1):24130.
13. Munshi RP, Joshi SG, Rane BN. Development of an experimental diet model in rats to study hyperlipidemia and insulin resistance, markers for coronary heart disease. Indian Journal of Pharmacology. 2014;46(3):270.
14. Jung K. Tietz Textbook of Clinical Chemistry and Molecular Diagnostics, Carl A. Burtis, Edward R. Ashwood, and David E. Bruns, editors. St. Louis, MO: Elsevier Saunders, 2006, 2448 pp., $229.00, hardcover. ISBN 0-7216-0189-8. Clinical Chemistry. 2006 Jun 1;52(6):1214-.
15. Nauck M, Graziani MS, Bruton D, Cobbaert C, Cole TG, Lefevre F, Riesen W, Bachorik PS, Rifai N. Analytical and clinical performance of a detergent-based homogeneous LDL-cholesterol assay: a multicenter evaluation. Clinical chemistry. 2000 Apr 1;46(4):506-14.
16. Dominiczak M, McNamara J. The system of Cardiovascular prevention. 103.125; Nauk M, Wiebe D, Warnick G. Measurement of High-Density-Lipoprotein Cholesterol. 221.244. handbook of lipoprotein testing (eds. Rifai, Warnick and Dominiczak),. 2000.